He-Ne激光血管内照射对健康兔红细胞变形性影响-第三军医大学学报.DOC

激光血管照射改善红细胞变形性的研究 邓 玲 陈仕国 马显光 （第三军医大学 生物医学工程与医学影像学院 物理学与生物物理学教研室；重庆400030) [摘要] 目的 用低能量He-Ne激光对成年大白兔进行血管内照射和血管外照射，对比研究红细胞变形指数的变化。方法 选成年大耳白兔21只，随机分成内照射组、外照射组和对照组，每组各7只。 照射组采用国产He-Ne激光器，分别作兔耳外周静脉血管内照射和外照射，每24小时照射一次，每次50min。照射前、照射一次后24h、照射7次后，每次各取静脉血检测红细胞变形指数。对照组正常对照饲养。检测在体血管对He-Ne激光的透过率。结果 ①内照射组和外照射红细胞变形指数均提高显著 (P0.05)。②活体血管组织对He-Ne激光的透过率为83.5%。结论 计算激光外照射与激光内照射的等效计量后，可用He-Ne激光血管外照射替代血管内照射，同样能提高红细胞变形能力，促进血液流动性，改善微循环。 关键词：He-Ne激光；血管照射；红细胞变形性 Study on hemal irradiation by laser to improve the erythrocyte deformability DENG Ling，CHEN Shi-guo，MA Xian-guang， (Department of Physics and Biophysics，College of Biomedical Engineering ＆ Medical Imaging. The Third Military Medical University，Chongqing 400038，China)[Abstract] Object To study the changes of erythrocyte deformability after irradiated the intravascular and extravascular by He-Ne laser. Method 21 dult albino rabbit have been randomly divided into three groups. Each group was 7. In irradiation groups, the He-Ne Laser has been used to irradiate the ear’s intravascular and extravascular of rabbits. Every 24 hours, the irradiation was conducted one time and each time, the irradiation was hold for 50min. The venous blood have been collected at the times of before irradiation, irradiated after first time and seventh time. The index of blood RBC deformation have been detected. The control group treated at a normal raising condition. Result ① In irradiation group, the index of RBC deformation increase significantly (P0.05). ② in vivo，the permeance rate of He-Ne Laser in vein is 83.5% . Conclusion After calculating equivalent power , the intravascular irradiation can been instead of extravascular irradiation, increase the erythrocyte deformability, improve the hemokinesis and minicirculation. [Key words] He-Ne laser； irradiation； erythrocyte deformability 据报道，低功率血管内照射能降低血液粘度，增强其流动性，改善微循环，临床上已用于治疗缺血性脑血管疾病等多种疾病[1] ～[4]。但由于血管内照射需要光针刺入血管，给病人带来痛苦，通常这类