临床型乳房炎奶牛与健康奶牛嗜中性粒细胞差异蛋白质组的表达-遗传.PDF

HEREDITAS (Beijing) 2012 10 , 34(10): 1298―1303 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2012.01298 临床型乳房炎奶牛与健康奶牛嗜中性粒细胞差异蛋白 质组的表达分析 1, 2 1 3 3 3 3 3 1 刘开东 , 柳楠 , 杜立新 , 魏彩虹 , 张莉, 路国彬 , 赵福平 , 刘积凤 1. , 266109; 2. , 266100; 3. , , 100193 嗜中性粒细胞是防止病原入侵的第一道防线, 且已有研究表明嗜中性粒细胞在奶牛乳腺免疫中发挥着 关键作用。文章运用双向凝胶电泳方法对临床型乳房炎奶牛与健康奶牛嗜中性粒细胞差异表达蛋白质组进行分 析, 成功获得分辨率较高、重复性较好的奶牛嗜中性粒细胞双向电泳凝胶图谱, 并通过MALDI-TOF MS 鉴定获 得差异表达的蛋白质7 种, 主要涉及细胞代谢、氧化应激、炎症反应等相关蛋白通路。实验获得的临床型乳房 炎奶牛与健康奶牛嗜中性粒细胞差异表达蛋白有望为今后奶牛乳房炎的抗病育种研究提供理论依据。 奶牛; 嗜中性粒细胞; 双向电泳; 蛋白质组学 Differential expression analysis of proteins in neutrophils between clinical mastitis and healthy dairy cows 1,2 1 3 3 3 3 LIU Kai-Dong , LIU Nan , DU Li-Xin , WEI Cai-Hong , ZHANG Li , LU Guo-Bin , 3 1 ZHAO Fu-Ping , LIU Ji-Feng 1. College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China; 2. Qingdao Institute of Animal Husbandry and Veterinary Medicine, Qingdao 266100, China; 3. National Center for Molecular Genetics and Breeding of Animal, Institute of Animal Science , Chinese Academy of Agricultural Sciences, Beijing 100193, China Abstract: Neutrophils provide the first line of defense against invading pathogens and have been reported to play a key role in bovine mammary immune. To examine the differential expression of proteins in neutrophils between clinical mastitis and healthy dairy cows, a 2-dimensional electrophoresis gel map with high repeatability was constructed for bovine neutro- phils. F