人中性粒细胞明胶酶关联脂质运载蛋白NGAL.PDF

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人中性粒细胞明胶酶关联脂质运载蛋白NGAL.PDF

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人中性粒细胞明胶酶关联脂质运载蛋白NGAL

武汉基因美科技有限公司 HumanNeutrophil Gelatinase AssociatedLipocalin (NGAL)ELISA Kit CatalogueNumber:JYM0253Hu  Forresearchuseonly.  For the quantitative in vitro determination of NGAL concentrations in Human serum, plasma, culture media or any biological fluid.  Expirationdate：sixmonths.  Storage： 2-8℃. Principle ThisELISA kit uses Sandwich-ELISA asthemethod.TheMicroelisa stripplateprovided in this kit has been pre-coated with an antibody specific to NGAL. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for NGAL is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain NGAL and HRP conjugated NGAL antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of450 nm.The OD value isproportional tothe concentration ofNGAL.You can calculate the concentration of NGAL in the samples by comparing the OD of the samples to thestandard curve. 地址：武汉市洪山区高新大道666号光谷生物城电话：027 1 网址： E-mail:jiyinmei027@163.com 武汉基因美科技有限公司 Materialsprovidedwiththekit Materialsprovidedwith 48determinations 96determinations Storage thekit 1 User manual 1 1 R.T. Closureplate 2 2 2 R.T. membrane 3 Sealedbags