低氧促进血管平滑肌细胞中jak基因表达-第三军医大学学报.docVIP

缺氧PC12细胞中JAK1/STAT1、3介导的信号通路的 赵吉清 董兆君 （第三军医大学预防医学系防化医学教研室，重庆400038） 【摘要】 目的 研究缺氧后PC12细胞JAK1、STAT1、STAT 3基因mRNA和蛋白表达的变化。方法 制备缺氧细胞模型。采用半定量RT-PCR技术和Western blot法检测缺氧对PC12细胞的JAK1、STAT1 、STAT 3基因mRNA和蛋白质的表达水平，并对PCR产物进行测序。 结果 缺氧后PC12细胞JAK1、STAT1、STAT 3mRNA和蛋白质表达水平升高，在缺氧后6h达最高，缺氧后24h的表达量低于12h，与对照组比较均有明显差别。RT-PCR产物直接测序证实扩增产物片断与Genbank中相应序列相同。结论 缺氧能增强PC12细胞中JAK1/STAT1,3基因的表达，在缺氧所致的脑损伤中可能发挥重要作用。 【关键词】 缺氧；PC12细胞；信号的转录因子和活化子1、3；Janus激酶1 Study on changes of JAK1/STT1,3 signal transduction pathway in PC12 cells Zhao Jiqing, Lin Hai, Wu Qiang, Li Yunpeng, Dong Zhaojun ( Department of chemical defence, Third military medical university, Chongqing 400038, China) 【Abstract】 Objective To study the effect of the exp?ession of JAK1 and STAT1,3 in PC12 cell line at hypoxia. Methods The cells were treated with hypoxia and the expression levels of JAK1,STAT1,3 mRNA and protein were detected by RT-PCR and Western Blot in PC12 cells. The products were sequenced by Sanger’s double strand DNA sequence determination. Results The expression levels of JAK1,STAT1,3 mRNA and protein were increased in PC12 cell and reached the highest level at 6h, decreased at 12h, but they were still higher than that of control group. It was shown that the sequences of amplification products by RT-PCR were the same to that in Genbank by sequencing. Conclusion Hypoxia reinforce the expressions of JAK1, STAT1 and 3 in PC12 cells. JAK1/STAT1 gene is possible to take the important action in brain injury. 【Key Words】 hypoxia; PC12 cells;JAK1;STAT1,3 基金项目：国家自然科学基金资助项目 作者简介：赵吉清（1970-），男，山东省牟平县人，副教授，医学博士，主要从事神经毒理方面的研究，发表论文15篇。电话：(023作者单位：400038 重庆 第三军医大学预防医学系防化医学教研室 通讯作者：董兆君，电话：(023JAK/STAT (Janus Kinase,JAK,蛋白酪氨酸激酶)/(signal transducer and activator of transcription, STAT,信号转导子与转录激活子)信号通路是一条应激反应通路,广泛参与细胞的增殖、分化成熟、凋亡以及免疫调节等过程,是众多细胞因子信号转导的重要途径之一。关于JAK-STAT的研究目前大多集中在造血系统、淋巴系统、肿瘤、肝脏疾病、造血系统疾病和心脏疾病，对其在神经系统的研究报道较少。必威体育精装版的文献表明，细胞因子及JAK-STAT途径不仅可能参与了脑的发育过程，而且参与了某些中枢神经系统疾病的病理过程，其中JAK1、STAT1和