长期服用龙胆泻肝汤对大鼠肝细胞色素p450的影响-嘉南药理大学.pdfVIP

理 龍 P450 類 ■ □ CNPH-91-01 行91 年1 1 91 年12 31 參 行 92 年 2 27 龍療 列龍 連 1gm/kg 量龍 列離 粒 RNA RNA ABC Rat UniversoChip 8K-1 列 RNA (labeling) cDNA (hybridization) GenePix 4000B [ 635 nm (Treatment) 532 nm (Reference)] GenePix Pro 3.0.5.56 療 率2 (up-regulation) 75理 beta-globin peptidylarginine deiminase type IV hemoglobin alpha chain hemoglobin alpha subunit lysozyme Phosphatidylinositol 4-kinase phosphatidylinositol-4-phosphate 5-kinas Thymosin beta-10 Topoisomerase II TPA Cathepsins Chloride channel protein (p64) farnesyl pyrophosphate synthase ubiquitin-homology domain protein (PIC1) cytoplasmic beta actin UDP-glucose dehydrogenase UDP-glucuronosyltransferase 率0.5 降 (down-regulation) 30 理 creatine kinase Heparin-binding growth associated protein NADH-ubiquinone oxidoreductase Syntaxin 2 龍 up-regulation down-regulation 理 連 P450 龍列 1 “Long-Dan-Shei-Gan-Tan”, a traditional Chinese herbal formulation, has been used for the treatment of hepatic-related diseases in the orient countries for more than thousand years. This study was aimed to primarily demonstrate the influence of long-term use of this formulation on the hepatic enzymes in rats by microarray biochip analysis. The preparation was given orally trice a day with the dosage of 1 gm/kg for consecutive seven days. Livers were dissected and the microsomes were prepared by a series of separating processes. RNA samples were extracted and purified from microsomes, and then the fluorescence-labeled probes were hybridized with the im