鲍曼不动杆菌喹诺酮类耐药基因突变分析.pdfVIP

DOI :10.13350/j.cjpb.2010.02.016 中 国 病 原 生 物 学 杂 志 2010 2 　5 2 ·86 · Journal of Pathogen Biology 　February 2010, Vol.5, No.2 · · 1 1 1 ＊ 2 1 3 ＊ 王敏 , 覃章顺, 李先平 , 杜世杰 , 曹虹 , 陈进伟 (1., 410011;2.2004 , 410013; 3., 410011) 【】　　, 。 　　 2002～2008 。2008 7～ 12 50 , PCR gyrA parC , (PCR- RFLP), PCR 。 　　 。50 62%, 46%。gyrA parC 305 bp400 bp 。PCR-RFLP , gyrA , 100% HinfⅠ, 53% HinfⅠ;parC , 29%HinfⅠ, 63% HinfⅠ。, gyrA parC , , HinfⅠGACTC GACTT, GANTC 。 parC , 89 GAA A G, parC 。 　　, 。gyrA parC 。 【】　;;gyrA;parC; 【】　R378.99　　　【】　A　　　【】　1673-5234(2010)02-0086-05 [Journal of PathogenBiology .2010 Feb;5(2):86-90.] Analysis of resistant gene mutations of quinolone in A ci netobact er ba umannii 1 1 1 2 1 3 WANG in , QIN Zhang-shun , LIXian-ping , DU Shi-jie , CAO Hong , CHEN Jing-wei 　(1.De- partment of Clinical Laboratory, TheSecond Xiangya Hospital of Central South University, Changsha 410011, Chi- na;2.Department of edical Laboratory of Class 2004, X iangya edical College of Central South University, Changsha 410013, China;3.Department of Immunology Rheumatology, TheSecond X iangya Hospital of Central South University, Changsha 410011, China) 【Abstract】　Objective　To investigate thedrug resistance of Acinetobacterbaumannii to quinolones and analyze its resist- ance to gene mutations.　Methods　Statistical data on drug-resistant A.baumanniiwere collected from 2002 to 2008.At the same time, 50 A.baumannii strains were collected at the Second Xiangya Hospital of Central