农业有机废物与城生活垃圾堆肥高温期微生物-湖南大学环境学院.docVIP

农业有机废物与城市生活垃圾堆肥高温期微生物种群结构比较 李 凤1,2 曾光明1,2* 范长征1,2 李 贞1,2 肖 勇1,2 (1. 湖南大学环境科学与工程学院长沙410082)(2. 湖南大学环境生物与控制教育部重点实验室 湖南 长沙 410082) 摘 要: 通过PCR、克隆文库方法分析了农业有机废物堆肥细菌种群的多样性。提取堆肥的DNA, 构建高温期的16S rDNA18S rDNA克隆文库, 结果表明: 农业有机废物16S rDNA克隆文库中分别共有个、个OTUs, 分别属于细菌域的1个、1个不同属, 18S rDNA克隆文库中分别共有个、个OTUs, 分别属于细菌域的个、个不同属, 推断农业有机废物的优势菌为Bacillus megaterium、Rhizobium sp、Phanerochaete chrysosporiumPenicillium sp. 同属或同种的菌株; 的优势菌为Bacillus megateriumAzospirillum sp.、Phanerochaete chrysosporium同种或同属的菌株。克隆文库细菌种群结构Comparison Microbial Communities During HighTemperature Period of Composting of OrganicAgriculture Wastes and Municipal Domestic Waste LI Feng1,2 ZENG Guang-Ming1,2* FAN Chang-Zheng1,2 LI Zhen1,2 XIAO Yong1,2 (1. College of Environmental Science and Engineering, Hunan University, Changsha, Hunan 410082, China)(2. Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Changsha, Hunan 410082, China) Abstract: Microbial diversity of composting of agriculture organic wastes (AOW) and municipal domestic waste(MDW) in the thermophilic(T) phase was analysed by Polymerase Chain Reaction(PCR) combined with constructing of clone library. The polymerase chain reaction-amplified 16S rDNA and 18S rDNA fragments with universal eubacterial primers(27F, 1495R) and fungal primers(EF4, Fung5) were subjected to constructing of clone library, and the results of clone library indicated: there were 18 OTUs and 21 OTUs in the 16S rDNA clone library of AOW and MDW composting sample, and they belonged to 14 and 15 different genuses respectively; in the 18S rDNA clone library of AOW and MDW composting sample, there were 8 OTUs and 9 OTUs, and they belonged to 8 and 9 different genuses respectively. The dominant microbes in the T phase of composting of AOW were related to Bacillus megaterium, Rhizobium sp., Phanerochaete chrysosporium, Penicillium sp., and the dominant microorganisms in the T phase of composting of MDW were related to Bacillus megaterium, Azospirillum sp