多穗柯挥发性组分分析及抗癌活性的初步研究-现代食品科技.pdfVIP

现代食品科技 Modern Food Science and Technology 2017, Vol.33, No.3 多穗柯挥发性组分分析及抗癌活性的初步研究 燕妮，王佐，高雄，张媛媛，林晓蓉，李斌，陈忠正 （华南农业大学食品学院，广东广州 510642） 摘要：本研究以多穗柯为材料，采用同时蒸馏萃取法（SDE ）提取挥发性组分，通过气质联用仪（GC-MS ）分析挥发性组分组 成，并以 Hepa1c1c7 小鼠肝癌细胞模型，以细胞存活率大于50%，诱导醌还原酶（QR ）活性等于或大于2 为活性指标，研究多穗柯 挥发性组分及其主要单体物质的抗癌活性。结果显示，GC-MS 共鉴定出 80 种多穗柯挥发性物质，占总挥发性组分相对百分含量的 92.56%，其中酮类、醇类和醛类物质是多穗柯挥发性组分的主要类型，其主要成分有香叶基丙酮、β-紫罗酮、壬醛和桉油烯醇等。抗 癌活性鉴定表明，多穗柯挥发性组分的浓度为(27.50±0.10) μg/mL 时可诱导QR 活性达到倍增，具有较强的抗癌活性；其中芳樟醇、 香叶基丙酮和壬醛三个主要挥发性单体成分诱导 QR 活性达到倍增的浓度分别为(54.53±0.10) μg/mL 、(283.10±0.10) μg/mL 和 (297.77±0.12) μg/mL。 关键字：多穗柯；挥发性组分；成分分析；抗癌活性 文章篇号：1673-9078(2017)3-35-39 DOI: 10.13982/j.mfst.1673-9078.2017.3.006 Analysis of the Volatile Components of Lithocarpus polystachyus Rehd. and a Preliminary Study of Their Anticancer Activity YAN Ni, WANG Zuo, GAO Xiong, ZHANG Yuan-yuan, LIN Xiao-rong, LI Bin, CHEN Zhong-zheng (South China Agricultural University, College of Food Science, Guangzhou 510642, China) Abstract: Lithocarpus polystachyus Rehd. was selected as a starting material to extract volatile components using simultaneous distillation extraction (SDE) method. The composition of volatile components was analyzed by gas chromatography–mass spectrometry (GC-MS). Subsequently, the anticancer activities of the volatile components and major monomeric substances of Lithocarpus polystachyus Rehd. were studied using mouse hepatoma cells (Hepa1c1c7). A cell viability of greater than 50% and quinone reductase (QR) induction activity greater than or equal to 2 were set as the activity indicato