响应面法优化蛋白酶菌株发酵条件.pdfVIP

248 2011, Vol. 32, No. 13 食品科学 ※生物工程 张 丹，闵伟红* ，刘景圣，解蕙铭，张 春，李锁霞，方 丽 (吉林农业大学食品科学与工程学院，发酵工程实验室，吉林 长春 130118) 目的：在单因素试验的基础上，应用响应面法优化可用于玉米高效浸泡的产蛋白酶菌株的发酵条件。方 法：通过单因素试验及响应面方法研究 C/N 、MgSO4 和 CaCl2 、接种量等因素对菌株产蛋白酶酶活力的影响，确 定发酵最佳工艺参数。结果：C/N 为 2 、MgSO4 0.5g/L 、CaCl2 0.2g/L 、接种量6% ，该蛋白酶酶活力最大值为 2504.8U/mL ，酶活力较优化前提高了4 倍。同时建立蛋白酶酶活力与 C/N 、MgSO4 添加量、CaCl2 添加量、接种 量之间的二次多项式模型，该模型可以很好地预测蛋白酶产量。结论：用响应面分析方法对该产蛋白酶菌株发酵 培养基进行优化，可获得最佳的工艺条件。 蛋白酶；玉米浸泡；优化；响应面 Response Surface Optimization of Fermentation Conditions for Protease Production by Pichia kudriavzevii Yeast ZHANG Dan ，MIN Wei-hong* ，LIU Jing-sheng ，XIE Hui-ming ，ZHANG Chun ，LI Suo-xia ，FANG Li (Fermentation Engineering Laboratory, College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China) Abstract ：In our previous work, we have isolated a protease-producing Pichia kudriavzevii yeast strain. This study was undertaken to use response surface methodology to optimize fermentation conditions for protease production by the strain. Protease activity in fermentation broth was investigated with respect to C/N ratio, MgSO4 and CaCl2 concentrations, and inoculum size. A quadratic polynomial model describing the effects of the fermentation conditions on protease production was established. The highest protease production of 2504.8 U/mL was achieved under the following conditions: C/N ratio of 2, 0.5 g/L MgSO4, 0.2 g/L CaCl2, and inoculum size of 6%, which was four times higher than before optimization. In addition, the established regression model was found to have good effectiveness in predicting protease production. Key words ：protease ；corn steeping ；optimization ；response surface 中图分类号：TS201.3 文献标识码：A 文章编号：1002-