一种可控的兔颈动脉切应力及ldl浓度极化模型王贵学1刘华1叶林.docVIP

一种可控的兔颈动脉切应力及LDL浓度极化模型 王贵学1, 刘华1, 叶林奇1, 李培培1, 向成平1, 何静2, 赵禾2 （1重庆大学生物工程学院， 2 重庆大学医院，重庆400044） 摘要：目的：建立一种可调控的切应力及低密度脂蛋白(low density lipoprotein, LDL)浓度极化动物模型,在体研究二者对动脉粥样硬化 (artherosclerosis ，AS)30%、40%、50%）(0.5%、1%、1.5%)40%狭窄度组最低；同一样本，左颈总动脉切应力高于右颈总动脉（P 0.05）AS病变，以40%狭窄度组病变更为显著。结论：兔颈总动脉硅胶管套扎模型是在体研究切应力及LDL浓度极化对AS影响的较好的动物模型。 关键词：动脉粥样硬化；切应力；低密度脂蛋白；浓度极化；动物模型 中图分类号: R363.1; R543.5; Q66 文献标识码：A A Controllable Wall shear Stress and LDL Concentration Polarization Model of Common Carotid Artery in Rabbit WANG Gui-Xue 1, LIU Hua1，YE Lin-Qi 1，LI Pei-pei 1，XIANG Cheng-Ping 1, HE Jing 2, ZHAO He 2（1 Bioengineering College of Chongqing University , 2 Hospital of Chongqing University ,Chongqing 400044，China)Abstract Objective: To establish a controllable wall shear stress (WSS) and low density lipoprotein(LDL) concentration polarization animal model to investigate effects 基金项目：教育部科学技术研究重点项目（NO：104158）；重庆大学2007年国家大学生创新性实验项目基金（CQUCX-G-2007-03）。 作者简介：王贵学(1963-), 男, 博士，教授、博导，从事心血管生物力学工程、动脉粥样硬化发病机制等研究。 刘华(1974-)，女，硕士，主治医师，从事动脉粥样硬化发病机制研究。 通讯作者：王贵学，电话：023 E-mail：wanggx@cqu.edu.cnof the two factors on formation of artherosclerosis(AS) in vivo. Methods: The left common carotid arteries (LCCA) of rabbits were operated a gel-silica pipe loop ligature operation with three different stenosis degree (30%, 40%, 50%) to generate different blood flow environment on local blood vessel, the right common carotid artery (RCCA) as a sham operation control. After operation the animals were fed three kinds of forages with three different cholesterol concentration (0.5%,1%,1.5%) to form different level of LDL concentration polarization, and general forage as a control. Hemodynamics parameters of ambi-CCA were detected by Color Doppler Flow Imaging at postoperative 8 weeks, then animals were killed to get ambi-CCA example for detection of pathology and Scanning Electron Microscope (SEM). Results: In the case of same stenosis degree, WSS of LCCA increase wit