中药活性小分子人工抗原合成的技术要点-天津中草药杂志社.pdfVIP

·1880 · 中草药 Chinese Traditional and Herbal Drugs 第43 卷 第10 期 2012 年10 月 中药活性小分子人工抗原合成的技术要点 1, 3 1, 2* 1, 2 1, 2 1, 2* 屈会化 ，赵 琰 ，李翼飞 ，杨爱玲 ，王庆国 1. 北京中医药大学“经典方剂的应用基础研究”创新团队，北京 100029 2. 北京中医药大学基础医学院，北京 100029 3. 北京中医药大学 科研实验中心，北京 100029 摘 要：中药免疫分析技术适用于中药复杂组分的研究，基于此技术的研究思路与方法越来越引起研究者的重视。免疫分析 的前提是抗体制备，中药活性小分子人工抗原的合成是中药活性成分抗体制备的关键步骤。从载体的选择、交联方法的选择、 纯化及鉴定方法、合成条件的优化及影响免疫原性的因素等方面，对中药活性小分子人工抗原合成的技术要点进行综述，从 而为中药免疫分析技术的建立和发展奠定基础。 关键词：中药活性小分子；人工抗原；免疫分析法；中药免疫分析；载体 中图分类号：R285 文献标志码：A 文章编号：0253 - 2670(2012)10 - 1880 - 06 Key techniques in artificial antigen synthesis of active small molecule in Chinese materia medica QU Hui-hua1, 3, ZHAO Yan1, 2, LI Yi-fei1, 2, YANG Ai-ling1, 2, WANG Qing-guo1, 2 1. “The Basic Research of Classic Recipe Application” Innovation Research Team, Beijing University of Chinese Medicine, Beijing 100029, China 2. School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China 3. Center of Scientific Research, Beijing University of Chinese Medicine, Beijing 100029, China Abstract: Immunoassay technology is suitable for the study on complex components in Chinese materia medica (CMM). Research ideas and methods based on this technology have drawn the increasing attention of researchers. The premise of the immunoassay is antibody preparation and the critical step of antibody preparation of active ingredients in CMM is the artificial antigen synthesis of active small molecule in CMM, of which the key technology is summarized in this article, including carrier choice, methods for cross- linking, purification and identification of artificial antigens, the optimization of the synthesis conditions as well as factors influencing the immunogenicity. This article is supposed to lay the foundation for the establishment and development of CMM immunoassay technology. Key words: active small mole