禽流感病毒NP蛋白单克隆抗体的研制与鉴定-预防兽医学专业毕业论文.pdf

扬州大学硕上学位论文 2 电泳蛋白条带反应，说明不是针对线性表位的．而可能是针对空间表位的单抗，其 具体表位还需进一步鉴定。这两株针对NP蛋白的单抗在禽流感病毒毒株的抗原分 析、禽流感血清学诊断和流行病学调查等方面存在着潜在的应用价值。 HI试验共同抗原 关键词：禽流感单克隆抗体间接ELISA Antibodies ofMonoeionai andIdentification against Preparation NPProteinofAvianInfluenzaVirus Qinghua M．S．Candidate：Huang Advisors：prof．Liu Xiufa。ll ofAnimal University,Jiangsu225009，China College Science、bterinaryMedicine，Yangzhou Abstract with micewereimmunized 200 The8-week-oldfemaleBALB／c intraperitoneally in influenzavirus Freundl u H9N2 avian emulsified subtype gformaldehyde-inactivated same emulsifiedinFretmd’s s weekslater,the completeadjuvant．Two antigen wereimmunized weeks same later,the incomplete intraperitoneaUy．Four adjuvant was three administered without afterfinal intravenously antigen adjuvant,anddays miceweDg with cellsfromimmunized fused immunization，spleen