Genetic Diversity of Tea Plant （Camellia Sinensis （L.） O.Kuntze） in Wuling Mountainous Area Revealed by ISSR Marker.pdf

茶 叶 JournalofTea 2013，39(4)：205—211 GeneticDiversityofTeaPlant(CamelliaSinensis(L．)O．Kuntze) inW ulingM ountainousAreaRevealedbyISSR M arker YueZhang，LiLu ，SaiyanYuan，XinPang，Wei—DongW ang， LinZhou，JohnKTanui，Xing—HuiLi TeaResearchInstitute，NamingAgriculturalUniversity，Naming210095，Jiangsu，China． #：Now workingindepartmentofteascienceandbiology，WuyiCollege，W uyishan，354300，China· ：Correspondingauthor：XHLi，e-mail：lxh@njau．edu．an Abstract Geneticdiversity and relationship of183 tea germplasmswereassessed by inter。simple sequencerepeat (ISSR)marker．ThestudyoptimizedtheISSR—PCRsystem forteaplants．Resultsshow thatgeneticsimilarity among183teaascensi0nswasbetween0．52-0．93，indicatingawidegenepoolandabroadgeneticvariationof Wulingtearesources．ThehighestsimilarityofaU materialsbetweenNolandNo2was0．93．Dendrogram ofthe 183 tea germplasmswere protracted by UPGMA clustering analysis alsorevealed the genetic diversity and relationshipoftheteagermplasmsofWulingmountainousarea．Besides，fingerprintofthe183teagermplasmswere constructed． Keywords Tea；germplasm ；ISSR；geneticdiversity． 1．IntrOductiOn Consumedworldwideasabeverage，theteaplant，CamelliasinensisL．，isoneoftheworldsmostimportant woody plantation crops． Besides， it also valued as a source of secondary metabolic products such as phytooxylipins… Originatedfrom China，thousandsofteavarietiesarewidelycultivatedin58countriesinAsia， ． Africa，Americas，OceaniaandEuropenow． Locatedatabout30 degreesofnorth latitude，andwith general altitudeof200—1200m，W ulingMountain belongstohumid subtropicalmonsoon climatearea_2]-Recentyears，in order to collectand protect tea germplasms，germplasm resourcesgardenhasbeenestablished inacademyofEntaiand133teagermplasmshave been saved． Itiswellknown thatknowledge ofthegeneticdiversity atintraspecific levelsisan important Drerequisitef0rfut