大赖草6-SFT基因的克隆及其转基因烟草抗旱和抗寒性分析.pdf

作物学报 ACTA AGRONOMICA SINICA 2016, 42(3): 389398 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2016.00389 大赖草6-SFT 基因的克隆及其转基因烟草抗旱和抗寒性分析 贺晓岚1 王建伟2 李文旭3 陈真真1 赵继新1 武 军1 王中华1 陈新宏1,* 1 / , 712100; 2 , 556011; 3 , 450002 : (6-SFT) RACE RT-PCR (Leymus racemosus, 2n = 4x = 28, NsNsXmXm) 6-SFT , 1863 bpLr-6-SFT (GenBank KT387273), 620 , , 6-SFT 6-SFT p1300-35SN-Lr-6-SFT , W38 PCR RT-PCR , , ; , , , Lr-6-SFT GH32 , : 6-SFT ; ; ; ; ; Cloning of 6-SFT Gene from Leymus racemosus and Analysis of Tolerance to Drought and Cold Stresses in Transgenic Tobacco 1 2 3 1 1 1 HE Xiao-Lan , WANG Jian-Wei , LI Wen-Xu , CHEN Zhen-Zhen , ZHAO Ji-Xin , WU Jun , WANG Zhong-Hua 1, and CHEN Xin-Hong1,* 1 Shaanxi Provincial Key Laboratory of Plant Genetic Engineering Breeding / College of Agronomy, Northwest AF University, Yangling 712100, China; 2 College of Environment and Life Science, Kaili University, Kaili 556011, China; 3 Wheat Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China Abstract: The fructan biosynthesis enzyme (6-SFT) plays an important role in plant in response to abiotic stresses. In this study, a full-length cDNA encoding sucrose