海岛棉转录因子基因GbMYB60的克隆、表达及其抗逆性分析.pdf

作物学报 ACTA AGRONOMICA SINICA 2016, 42(9): 13421351 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2016.01342 海岛棉转录因子基因 GbMYB60 的克隆、表达及其抗逆性分析 高 巍** 刘会利** 田新权 张 慧 宋 洁 杨 勇 龙 璐 宋纯鹏* / / , 475004 : MYB , 7124 MYB , , GbMYB60990 bp, 36.9 kD R2R3 MYB GbMYB60 GbMYB60 , , NaCl , GbMYB60 , GbMYB60 , ; GbMYB60 : ; MYB ; ; ; Cloning, Expression, and Functional Analysis of Transcription Factor Gene GbMYB60 in Cotton ** ** GAO Wei , LIU Hui-Li , TIAN Xin-Quan, ZHANG Hui, SONG Jie, YANG Yong, LONG Lu, and SONG * Chun-Peng School of Life Science, Henan University / State Key Laboratory of Cotton Biology / Henan Key Laboratory of Plant Stress Biology, Kaifeng, 475004, China Abstract: MYB transcription factors are conserved proteins in all eukaryotic cell, which play important roles in plant growth, development, metabolism, abiotic and biotic stress resistance, as well as phytohormone-mediated signal transduction. In this re- search, a MYB gene was isolated from the sea-island cotton cultivar Hai 7124. This gene was named GbMYB60 based on the se- quence similarity search and phylogenetic analysis. The full-length of GbMYB60 coding sequence is 990 bp, and GbMYB60 en- codes a 36.9 kD R2R3-type MYB protein, which is specifically