脂肪酶的固定化及其在食品领域的应用-中国农业科学院.pdfVIP

生物技术通报 ·综述与专论· BIOTECHNOLOGY BULLETIN ( ) 2016, 32 11 :59-64 脂肪酶的固定化及其在食品领域的应用 魏雪  孙丽超  李淑英  王凤忠  辛凤姣 （中国农业科学院农产品加工研究所，北京 100193） 摘 要 ： 脂肪酶 （lipase，EC ）是一类能将长链脂肪酸甘油酯水解成脂肪酸和单甘油酯、甘油二酯或甘油的生物酶， 主要来源于动物、植物和微生物。脂肪酶在食品领域的应用有巨大潜能，改善脂肪酶的性能对于实现工业上连续化和低成本的生 产具有重要的意义。固定化酶技术可以通过提高脂肪酶的酶活力、稳定性及回收率显著改善脂肪酶的性能。固定化脂肪酶在食品 领域中被广泛应用于糖酯合成、油脂改性、芳香味酯类化合物及抗坏血酸酯类抗氧化剂的合成。介绍了脂肪酶及固定化酶技术， 并综述了近7 年固定化脂肪酶在食品领域中的应用。最后对如何提高脂肪酶性能进行了展望。 关键词 ： 脂肪酶 ；固定化脂肪酶；食品领域应用 DOI ：10.13560/ki.biotech.bull.1985.2016.11.008 Immobilization of Lipase and Its Application in Food Industry WEI Xue SUN Li-chao LI Shu-ying WANG Feng-zhong XIN Feng-jiao （Institute of Food Science and Technology in CAAS ，Beijing 100193 ） Abstract: Lipase（EC ）is a kind of enzyme that can hydrolyze long chain fatty acid esters into fatty acid and monoglyceride， diglyceride or glycerol，and it is mainly from animals，plants and microorganisms. Considering the great application potential of lipase in the food industry，optimization of lipase performance is of significance for achieving continuous and low-cost production. Immobilized enzyme technology significantly enhanced the lipase performance by increasing the enzymatic activity，stability and the recovery rate. Therefore， immobilized lipase is widely used in the food industry，including the enzymatic synthesis of glycolipids，oil modification，and the synthesis of aromatic ester compounds and ascorbyl esters. This article introduces the lipase and the immobilized enzyme technology，and mainly reviews the application of immobilized lipase in the food industry in last seven years，finally prospects how to improve the performance of lipase. Key words: lipase