时间分辨免疫荧光与化学发光法.doc

时间分辨免疫荧光与化学发光法、酶联免疫法测定乙肝标志物的比较 摘? 要 目的: 比较化学发光免疫测定（CL）、酶联免疫（EIA）与时间分辨免疫荧光（TRFIA）三种方法检测乙肝病毒免疫标志物的优缺点。方法: 用上述三种方法的检测试剂及仪器对189例临床血清标本分别进行检测，然后进行结果分析。结果: 三者对乙肝表面抗原的检测灵敏度均达到了0.1ng/ml。对HBcAb的测定，以CL的灵敏度最高，ELISA最低。对表面抗体、E抗原及E抗体的检测，相互符合率均在95%以上，但对核心抗体的检测符合率， TRFIA与CL为83.23%，TRFIA与EIA为85.19%。结论: 三种方法的检测性能相差不大，但操作性各有特点，应按各实验室具体情况加以选择。　　关键词：化学发光法，酶联免疫试验，时间分辨荧光免疫测定　　本文作者:肖征 周薇薇　白立彦　赵莉萍?? Comparison of chemiluminescence, ELISA and time-resolved fluoroimmunoassay in detecting Hepatitis B markers  肖征，副主任医师，副教授周薇薇，主管技师白立彦，主管技师赵莉萍，主管技师解放军总医院微生物科，100853 北京Zheng Xiao, Weiwei Zhou, Liyan Bai, Liping ZhaoGeneral Hospital of PLA, Beijing, 100853 PRC ? Abstract Subject: To compare three deferent assays to see their abilities in detecting hepatitis B markers. 　　Methods：By using Chemiluminescence (CL), ELISA and Time-resolved fluoroimmunoassay (TRFIA), 189 serum samples were tested for hepatitis B markers, then the results were analyzed.　　Results: The three assays all can detect as low as 0.1ng/ml of HBsAg. But in HBcAb, The CL showed the highest sensitivity, ELISA showed the lowest sensitivity. The coincidences of the three methods in detecting HBsAb, HBeAg, and HBeAb are normally above 95%, but for HBcAb, the coincidence was 83.23% between TRIF and CL, and 85.19% between TRIFA and EIA. 　　Conclusion: There were not much difference among these three methods regarding their abilities in detecting hepatitis B markers, but the maneuver flexibilities of each method differed a lot. One should consider his own labs situation before making choice.?? 　　Key words: chemiluminescence, ELISA, time-resolved fluoroimmunoassay ? 自二十世纪七十年代以来，许多高灵敏度的测定方法应用于临床免疫学检测。其中，酶联免疫法应用最广。近年来发展起来的CL与TRFIA测定据称是灵敏度非常高的方法[1,2,3]。本文就这三种方法对乙肝免疫标志物的测定作一比较，以下结果供大家参考。 ? 材料与方法 1.试剂与器材： 时间分辨免疫荧光测定为上海新波生物公司生产的乙肝两对半测定试剂盒，及由该公司提供的洗板机、孵育器和时间分辨免疫荧光测定仪以及分析判定结果软件系统。　　酶联免疫方法试剂为SORIN公司（HBsAg）、科华公司（HBsAb、HBeAg、HBeAb、HBcAb）产品； 仪器有洗板机（WELLWASH4）、酶标比色仪（READER 230S），部分试验在FAME（全自动开放式酶免分析仪）上进行。　　化学发光法为强生公司Vitros Eci化学发光免疫测定