链霉菌无痕敲除方法研究进展-微生物学报.pdfVIP

1100 谷燕燕 等/链霉菌无痕敲除方法研究进展 生 物 工 程 学 报 Chinese Journal of Biotechnology August 25, 2013, 29(8): 1100−1112 /cjbcn ©2013 Chin J Biotech, All rights reserved 综 述 链霉菌无痕敲除方法研究进展 谷燕燕，耿伟涛，宋存江 南开大学生命科学学院 分子微生物与技术教育部重点实验室，天津 300071 谷燕燕, 耿伟涛, 宋存江. 链霉菌无痕敲除方法研究进展. 生物工程学报, 2013, 29(8): 1100−1112. Gu YY, Geng WT, Song CJ. Progress in markerless knockout of genes on chromosome of Streptomycetes . Chin J Biotech, 2013, 29(8): 1100−1112. 摘 要: 链霉菌是革兰氏阳性放线菌，能产生大量具有重要价值的天然代谢产物。随着基因测序技术、分子 生物学和合成生物学的不断发展，人类对链霉菌家族有了更深的了解，从分子水平对其基因组进行改造的手段 也越来越多。通过对链霉菌基因组合理、高效的精简，将提高链霉菌代谢产物的产量和质量，降低底物原料的 消耗量。无痕敲除就是开展此项研究工作的重要手段。文章综述了近年来在链霉菌中广泛使用的分子操作方法 并重点对链霉菌无痕敲除方法进行了总结。 关键词: 链霉菌，无痕敲除，位点特异性重组，归巢核酸内切酶 Progress in markerless knockout of chromosome genes in Streptomycetes Yanyan Gu, Weitao Geng, and Cunjiang Song Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China Abstract: Streptomycetes are Gram-positive bacteria of Actinomycetales. These organisms can produce many economically important secondary metabolites. With the development of molecular biology, gene sequencing technology and synthetic biology, people gained a better understanding of the Streptomyces family. The means to transform genome on the molecular level is also increasing. By simplifying the Streptomyces genome rationally and efficiently, it will improve Received: March 12, 2013; Accepted: April 19, 2013 Supported by: National Basic