濒危特有种掌叶木的微卫星遗传多样性研究-广西植物.docVIP

濒危特有种掌叶木的微卫星遗传多样性研究 滕婕华1，卫家贤1，李象钦2，李超群3，倪敏1，翁乐逸1，谢国文1* 广州大学生命科学学院 ，广东 广州 510006；2. 广西植物研究所，广西 桂林 541006；3.中国科学院植物研究所，北京 100093. 摘要: 利用9对微卫星(SSR)分子标记来揭示掌叶木（Handeliodendron bodinieri）的遗传多样性, 结果表明0.299, P＜0.05）。(SSR), 濒危植物, 遗传多样性, 毛细管电泳 Endangered species endemic to microsatellite genetic diversity study on Handeliodendron bodinieri TENG Jie-hua1, WEI Jia-xian1, LI Xiang-qin2, LI Chao-qun3,NI Min1,WENG Le-yi1, XIE Guo-wen1* （1. College of Life Sciences, Guangzhou University, Guangzhou 510006, China; 2. Guangxi Institute of Botany, Guilin 541006, China, 3. Institute of Botany, Chinese Academy of Sciences, Beijing 100093） Abstract: Genetic diversity of Handeliodendron bodinieri was studied with microsatellite (SSR) markers. By?using?9 pairs polymorphic microsatellite loci ?to?reveal?H.?bodinieri was?rich?in?genetic?diversity. Average number of alleles （Na） and effective number of alleles (Ne) were 3.903 and 2.545 respectively. The mean expected heterozygosity (He) was 0.521 and Shannon’s diversity (I) was 0.962，PIC=0.465. Natural populations of H. bodinieri had relatively high level of genetic diversity, however the genetic diversity of the populations were reduced due to because of sabotage etc. The majority of genetic variation occurred within populations. The genetic distance among 8 populations of H. bodinieri was between 0.032 and 0.164. According to genetic distance UPGMA, genetic differentiation (Gst = 0.027), GstN=0.031, GstH=0.064,gene flow (Nm) was 3.368. Results analyzed by AMOVA showed that the variation among the populations was 3%，the variation within the populations was 97%. Mantel test revealed that there was positive correlation between genetic distance and geographical distance（r=0.299, P＜0.05）among the populations. The results provide a scientific basis to develop valid strategies for the protection the biodiversity of H. bodinieri. Key words: Handeliodendron bodinieri, microsatellite(SSR) , Endangered plant, genetic diversity, capillary electroph