1-s20-(早期饲养水平对妊娠的影响)(血样的储存)S03.pdf

Animal Reproduction Science 80 (2004) 341–352 Changes in feeding level during early pregnancy affect fertility in gilts J.V. Virolainen a,∗, A. Tast a , A. Sorsa a , R.J. Loveb , O.A.T. Peltoniemi a a Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Helsinki, Pohjoinen Pikatie 800, Saarentaus 04920, Finland b Department of Clinical Veterinary Sciences, Faculty of Veterinary Science, University of Sydney, Camden, NSW 2570, Australia Received 24 February 2003; received in revised form 15 July 2003; accepted 13 August 2003 Abstract Modified feeding combining the benefits of restricted feeding after ovulation and abundant feed- ing during implantation in autumn was tested. Three groups of eight gilts were housed with indi- vidual feeding stalls and fed 40 MJ per day of a commercial ration. Following insemination gilts were fed 27 MJ per day (LLL) or 54 MJ per day (HHH) for 34 days or 27 MJ per day for 10 days, 54 MJ per day for 7 days followed by 27 MJ per day until day 34 (LHL). Blood for progesterone analysis was collected daily during the week of ovulation and then twice a week until the end of the study. For LH assay, blood was collected from five gilts from each group at 15 min interval for 10 h on the day 15 of pregnancy. Gilts were weighed three times at intervals of 4 weeks. The effect of dietary treatment was significant (P 0.05) on body weight gain from days 0 to 30 of pregnancy, 1201, 287 and 438 g per day for groups HHH, LLL and LHL respectively. The pregnancy rate at day 34 was significantly higher (P 0.005) in HHH-group (100%) compared with LLL (25%) and LHL (38%) although HHH group had significantly lower (P 0.05) progesterone concen- tration on days 9 and 12. The basal LH level wa