不同继代次数对酿酒葡萄黑比诺植株性状和移栽成活率的影响.pdf

World Journal of Forestry 林业世界, 2014, 3, 42-47 Published Online July 2014 in Hans. /journal/wjf /10.12677/wjf.2014.33008 The Effect of Various Subculture Times on Plant Traits and Transplanting Survival Rate of Wine Grape Cultivar “Heibinnuo” 1 1 2 1 1 1 Fenghong Lang , Jinzhu He , Huiping Guo , Guihe Yang , Yanqin Ren , Bin Zhou , Hairong Huang1, Lei Zhang1 1 Ningxia Grape Plantlet Engineering and Technology Research Center, Yinchuan 2 Nningxia State Farm Xixiawang Industry Co., Ltd., Yinchuan Email: langfh2011@163.com rd th th Received: Jun. 23 , 2014; revised: Jul. 20 , 2014; accepted: Jul. 25 , 2014 Copyright © 2014 by authors and Hans Publishers Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). /licenses/by/4.0/ Abstract In this study, test-tube plantlets of three vegetatively propagated lines from wine grape cultivar “Heibinnuo” were selected as plant materials to investigate the effects of subculture times on plant traits and transplanting survival rate of various vegetatively propagated lines. The results indi- cated that with the increase of subculture times and subculture prolonging three vegetatively propagated lines from wine grape cultivar “Pinot Noir” maintained the original plant traits of the cultivar in leaf shape, leaf color and shoot buddy etc. Subculture times have no effects on plantlet rate and survival rate. Therefore, we proposed that “cutting culture method” does not affect mi- cro-propagation characteristics and plant life vigor of wine grape cultivars. Keywords