低氧促进间充质干细胞成骨相关活性.doc

缺氧微环境促进骨髓间充质干细胞成骨相关活性的初步研究 徐凌1，黄姣2 1重庆医科大学附属口腔医院修复科，重庆，中国。2重庆医科大学附属口腔医院牙周科，重庆，中国。 摘要 目的：间充质干细胞由于其特有的成骨生物性，因而在骨组织工程领域中备受关注。了解在一些生理微环境，如，缺氧环境在间充质干细胞相关骨再生中发挥的作用。 方法：取成年新西兰雄兔髂嵴内侧骨髓腔骨髓1ml------以培养骨髓间充质干细胞，分别在常氧（含有5%二氧化碳的空气中）和缺氧（含5%二氧化碳和95%氮气）中培养16小时，然后分别通过放射定量法检测氚标记胸腺嘧啶核苷（3H-TdR）细胞掺入率来检测MSCs 的DNA合成情况以检测两组MSCs增值情况；碱性磷酸酶黄色试剂盒检测两组碱性磷酸酶（alkaline phospatase， ALP）活性；ELISA检测两组1型胶原和3型胶原合成情况。 结果：3H-TdR在缺氧组明显高于正常组（p＜0.05）；ALP活性在缺氧组明显高于正常组（p＜0.05）。1型胶原和3型胶原在缺氧组明显高于正常组（1型胶原p＜0.05， 3型胶原 p＜0.01）。 结论：缺氧微环境可能对MSCs增值和成骨相关活性有促进作用。 关键词：缺氧；骨髓间充质干细胞；增值；成骨；组织工程 中图分类号 R781.4 通讯作者：黄姣 重庆医科大学附属口腔医院牙周科 【基金项目】 重庆市科委自然科学基金面上项目（CSTC 2011jjA0274） 通讯作者 黄姣 Hypoxia Induces Osteogenesis-Related Activities in Mesenchymal Stem Cells-Pilot Study Huang Jiao1， Xu Ling2 1 Department of Periodontology， the Affiliated Hospital of Stomatology，Chongqing Medicval University，Chongqing，400015，P.R.China 2 Department of Prosthotics， the Affiliated Hospital of Stomatology，Chongqing Medicval University，Chongqing,400015，P.R.China Abstract Objective：Mesenchymal stem cells (MSCs) have received much attention in the field of bone tissue engineering due to their biological capability to differentiate into osteogenic lineage cells. In the present study, we investigated the effects of hypoxia on biological capability of MSCs. Methods：MSCs were isolated from adult rabbit bone marrow, and were cultured in vitro under normoxia(air with 5%CO2) or hypoxia (5% CO2 and 95% N2). The proliferation of MSCs, alkaline phospatase (ALP) activity, and production of collagens type Ⅰ and type Ⅲ (ColⅠ/ Ⅲ ) were examined. Results：In the present study ，we found that hypoxia significantly induced the proliferation of MSCs and increased ALP activity and the production of ColⅠ/ Ⅲ. Conclusions：Our results indicate that hypoxia enhances MSCs to differentiate into osteogenic lineage cells. Keywords：hypoxia ，MSC，proliferation ，Osteogenesis ，tissue engineering Supported by Natural Science Foundation Project of CQ .CSTC（2011jjA0274） 间充质干细胞由于其特有的成骨生物