柑桔黄龙病诊断法的研究进展-福建农业学报.PDF

Fuj ian J ournal of A gricultural S ciences 1 1 2 田亚南　柯　穗　柯　冲 1 2 ( , 08855; , 350003) , , , , , 70 ( BLO) , , , 80 , BLO , , , , , , , DNA P1 P2 P1 P DNA P PCR ( ) Sout her 2 3 PCR , ; - ( ) , Q PCR ; ; ; ; S 436. 66 T he Progress on Diagnosis and Detection of Cit rus Huanglongbing Tia Ya a , Ke Sui a d Ke Cho g ( 1 Rutg ers University , New Jersey 08855, USA 2 Fuj ian A cademy of Ag ricultural Sciences, Fuz hou 350003, China) Abst ract Diag osis a d det ect io are importa t aspect of citrus Hua glo gbi g research. I the , , , past diag osis of citrus Hua glo gbi g was mai ly based o its sympomatology amely yellow shoot a d mottle leaf. Duri g 1977- 1978, the causative bacterium like orga ism ( BLO) was fou d i sieve cells of diseased leaf u der electro microscope. Aft er the , the electro microscope has . bee used as a major t ool for diag osis of citrus Hua glo gbi g disease A improveme t came about w he it w as fou d that the BLO ca be t ra smitt ed from citrus to periw i kle by dodder a d partially purified by differe t ial ce t rifugatio , which made it possible to prepare a tisera, both mo oclo