不同炮制工艺对款冬花中的款冬酮影响的研究.pdfVIP

目 录 摘要 Ⅰ- Ⅱ 1 前言1 2 材料与仪器3 2.1 材料3 2.2 仪器3 3 方法与结果4 3.1 蜂蜜的炼制4 3.2 甘草水的煎煮4 3.3 正交试验因素4 3.4 款冬花的炮制5 3.5 醇溶性浸出物5 3.6 款冬酮的提取与含量测定5 4 结果7 4.1 方法学考察结果分析7 4.2 蜜炙款冬花结果分析10 4.3 甘草制款冬花结果分析11 5 讨论12 5.1 提取方法的确定12 5.2 流动相比例的确定12 5.3 不足之处12 参考文献13 综述14 致谢21 附录A 蜜炙款冬花样品高效液相色谱图22 附录B 甘草炙款冬花样品高效液相色谱图26 不同炮制工艺对款冬花中款冬酮影响的研究 摘要：目的 对不同炮制工艺的款冬花中的款冬酮的影响进行研究，优化款冬花的炮制工艺。 方法 通过正交试验方法，以款冬酮、醇溶性浸出物为考察指标，优选款冬花的炮制工 艺。结果 蜜炙款冬花最佳工艺为：加蜂蜜的量为30%，炒制时间为11min，闷润的时间为3h ，甘草制款冬花最佳工艺为：甘草用量为6%，浸泡时间为14h，烘干温度为70℃。 结论 本实验工艺简单，稳定可行，材料易得，可用于款冬花的工业化生产。 关键字：款冬花；款冬酮；炮制工艺；醇溶性浸出物 Different processing technology on the research on the effects of coltsfoot ketone coltsfoot flower Abstract: objective this topic in the theory of traditional Chinese medicine and traditional Chinese medicine theory,under the guidance of the different stir-baked process coltsfoot ketone of coltsfoot flower were studied,the influence of optimizing the processing technology of coltsfoot flower. Methods the orthogonal test method and the method of multi-index comprehensive evaluation,the processing technology of determining coltsfoot flower. First of all,according to the orthogonal experiment design processing coltsfoot flower,then extracted by the method of ultrasonic extraction coltsfoot ketone,then using high performance liquid chromatography (HPLC) to determine the coltsfoot ketone content; Second is according to the 2010 edition of China pharmacopoeia method in each group after processing the coltsfoot flower alcohol soluble extract,and its content is calculated,finally integrated the two measures,it is concluded that the best processing technology. Results honey coltsfoot flower optimum process for: honey 20%,making time for 6 min,boring time for 2 h,licorice coltsfoot flower optimum process is: licorice dosage was 7%,the soaking time for 14 h,drying temperature is 70 ℃. Conclusion this stu