天然抗氧化剂与自由基.ppt

报告人：何山 2006-03-12 Mitochondrial Biogenesis in Mammals: The Role of Endogenous Nitric Oxide 天然抗氧化剂对自由基损伤的干预 天然抗氧化剂的分类 内源性：SOD、谷胱甘肽过氧化氢酶 维生素：VE、VC、β－胡萝卜素 植物天然产物：多酚类物质、多糖 微量元素：铜、锌、硒 Resveratrol protects rat hearts from ischemia reperfusion injury Thioredoxin, a Singlet Oxygen Quencher and Hydroxyl Radical Scavenger Hydroxyl radicals, Fenton reaction. Singlet oxygen, photolysis of rose bengal singlet oxygen quenching effect Cysteine is important for the reactive oxygen scavenging properties Curcumin is a singlet oxygen quencher ESR study on the structure-antioxidant activity relationship of tea catechins and their epimers Superoxide anion assay Singlet oxygen assay Assay for the carbon-centered free radicals from AAPH DPPH radical assay Singlet oxygen assay Assay for the carbon-centered free radicals from AAPH DPPH radical assay Antioxidant activity of caffeic acid 2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging DPPH scavenging total antioxidant activity by ferric thiocyanate method total reductive capability using the potassium ferricyanide reduction method superoxide anion radical scavenging metal chelating activities Scavenging effects of tea catechins and their derivatives on DPPH Effect of galloyl group ortho-dihydroxyls in the B ring is important Effects of pH and DPPH radical scavenging ability of tea catechins Protective effect of green tea polyphenols on the SH-SY5Y cells against 6-OHDA induced apoptosis through ROS–NO pathway Assessment of cell viability Morphological changes Flow cytometric analysis using Annexin V and PI Measurement of intracellular ROS Measurement of mitochondrial membrane potential Intracellular calcium concentration Measurement of intracellular NO Western blot for nNOS and iNOS Measurement of protein-bound 3-NT Assay for ROS generation during the autooxidation process of 6-OHDA ESR assay for p-quinone free radical during the autooxidation process of 6-OHDA Antioxidant Effects of Resveratrol and its