药学毕业论文--维药家独行菜子镇咳、抗炎及抑菌作用初探.doc

维药家独行菜子镇咳、抗炎及抑菌作用初探 作者：包晓玮，周旋 郭庆勇 韩海霞 阿依姑丽 舒云芳 王颖 【摘要】 目的研究维吾尔族传统药材家独行菜子水提物的镇咳、抗炎作用及其水提物、醇提物的抑菌作用。方法以不同溶剂分离家独行菜子各组份，并以氨水引咳法探讨其水提物的镇咳作用;同时以二甲苯致炎法探讨其水提物的抗炎作用;通过抑菌实验探讨其水提物、醇提物对大肠杆菌、枯草芽孢杆菌、铜绿假单孢菌、金黄色葡萄球菌的抑菌作用。结果家独行菜子水提物组与生理盐水组相比，能明显延长咳嗽潜伏期、降低每分钟咳嗽次数，及抑制鼠耳肿胀作用(均Plt;0.05);且家独行菜子水提物、95%乙醇提物、甲醇提物对4种细菌均有明显的抑菌作用。结论家独行菜子水提物具有显著的镇咳、抗炎作用，水、醇提物均具有明显的抑菌作用。 【关键词】 家独行菜子; 镇咳; 抗炎; 抑菌 　　Abstract：ObjectiveTo study the antitussive and anti-inflammatory effects of water extract of Semen Lepidii Sativi, an Uyghur traditional medicine and the bacteriostatic effect of its water extract, alcohol extract. MethodsTo separate different components from Semen Lepidii Sativi with different solvent. The antitussive effect of water extract was observed in the cough model induced by ammonia,and also the anti-inflammatory action was tested in the inflammatory model induced by Xylene.The bacteriostatic effect of the water extract, alcohol extract were studied on E.coli, Bacillus subtilis, Pseudomonas bacilli and Staphylococcus aureus.ResultsWater extract of Semen Lepidii Sativi could delay latent period, reduce number of cough, and inhibit inflammation of mouse ear (Plt;0.05).The bacteriostatic effect of water extract , methanol extract and ethanol extract were appear to four strains of bacteria. ConclusionThe water extract of Semen Lepidii sativi has antitussive and anti-inflammatory effects and both of water extract, alcohol extraction has obvious effect Bacteriostatic. 　　Key words：Semen Lepidii sativi; Antitussive; Anti-inflammatory; Bacteriostatics 　　家独行菜子(Semen Lepidiμm Sativi)是十字花科、独行菜属植物家独行菜Lepidiμm sativμm L.的干燥成熟种子，是维吾尔民族传统药物之一，维吾尔药名为台尔台孜Tertiz[1]。据文献报道本品有扩张冠状动脉、解毒、理肺杀虫、广谱抗菌、利尿助阳、促进伤口愈合等作用。可行水消肿、镇咳平喘、健脾胃，用于治疗气管炎、消化不良、腹泻及伤口不愈等疾病[2，3]，但其具体的有效成分及药效作用很少见报道。前期仅探讨了家独行菜子水浸液的抗实验性胃溃疡、促进溃疡愈合、抑制小肠运动及镇痛作用[4]。本文对家独行菜子各组份进行初步的分离，探讨其水提物的镇咳、抗炎作用，并观察其水提物、醇提物的抑菌作用，以期为家独行菜子的进一步研究提供依据。 　　1 材料与仪器 　　1.1 实验材料动物：ICR小鼠60只(清洁级)，♀♂兼用，体重(20±2) g，由新疆医科大学实验动物中心提供。受试药物：家独行菜子(Semen Lepidiμm Sativi)原植物药用种子部分，购于维吾尔药材市场。实验病菌：