计算机在生物学中的作用期末试题.doc

鲁东大学 生命科学学 学院20 11－20 12学年第 1 学期 《 计算机技术在生物学中的应用 》课程论文 课程号：2512290-200 论文题目： 1、请以E.coli heat-labile enterotoxin B subunit 基因在转基因植物中高效表达为研究题材，查询相关基因序列，相关中外文文献、结合已学知识，以其中1篇中文文章的部分内容为素材，运用Endnote，以planta期刊为模板，书写论文。 2、鼓励个性发展，也可不局限上述研究题材。 论文要求：（对论文题目、内容、行文、字数等作出判分规定。） 基因序列查询（5分），中外文文献查询（10分）。 论文目录自动生成（10分），参考文献中外文（10分），两图片合理放在一起（15分）、论文引用（10分）、论文结构合理（15分）、正确运用多种生物软件（20分）。 统一上交以本课程论文模板为标准的论文。打印的论文除上交一份纸质论文外，还需以学号+姓名为标题向qiushengzhang001@126.com发电子版。（5分） 教师评语： 教师签字： 年 月 日 正文 Oral Vaccination of Baculovirus-Expressed VP28 DisplaysEnhanced Protection against White Spot Syndrome Virusin Penaeus monodon Syed Musthaq S1, Jimmy Kwang1,2* Animal Health Biotechnology, Temasek Lifesciences Laboratory, National University of Singapore, Singapore, Singapore, 2 Department of Microbiology, Faculty ofMedicine, National University of Singapore, Singapore, Singapore Abstract White Spot Syndrome Virus (WSSV) is an infectious pathogen of shrimp and other crustaceans, and neither effectivevaccines nor adequate treatments are currently available. WSSV is an enveloped dsDNA virus, and one of its major envelopeproteins, VP28, plays a pivotal role in WSSV infection. In an attempt to develop a vaccine against WSSV, we inserted theVP28 gene into a baculovirus vector tailored toexpress VP28 on the baculovirus surface under the WSSV ie1 promoter (Bac-VP28). The Bac-VP28 incorporated abundant quantity (65.3 mg/ml) of VP28. Shrimp were treated by oral and immersion vaccination with either Bac-VP28 or wild-type baculovirus (Bac-wt). The treatment was followed by challenge with WSSVafter 3 and 15 days. Bac-VP28 vaccinated shrimp showed significantly higher survival rates (oral: 81.7% and 76.7%;immersion: 75% and 68.4%) than Bac-wt or non-treated shrimp (100% mortality). To verify the protective effects of Bac-VP28, we examined in vivo expression of VP28 by immunohistochemistry and quantified the WSSV copy numbe