四逆散对疲劳大鼠学习记忆及脑内GABA_B1表达的影响.pdfVIP

现代生物医学进展 Progress in Modern Biomedicine Vol.11 NO.1 JAN.2011 · 5 · 四逆散对疲劳大鼠学习记忆及脑内GABA_B1 表达的影响* 刘 燕 刘晓兰 宋月晗 范新六 马佳美 王 霁 许展嘉 李 峰△ 100029 （北京中医药大学 北京 ） 摘要 目的：探讨四逆散对疲劳大鼠学习记忆及脑内GABA_ B1 受体的影响。方法：应用游泳训练和睡眠剥夺方法复制疲劳动物 Y- GABA_ B1 模型，运用 迷宫检测各组大鼠的学习记忆能力，采用免疫组化方法检测大鼠脑内 受体阳性反应物的变化。结果：模 Y- GABA_ B1 型组与正常组相比，大鼠 迷宫正确反应率明显下降，错误反应率明显升高，大鼠脑内 的阳性反应物数目明显增多； 中药治疗组与模型组相比，正确反应率明显升高，错误反应次数明显下降，脑内GABA_ B1 受体的阳性反应物数目明显减少。结 论：四逆散对疲劳大鼠学习记忆能力的下降具有改善作用。 GABA _B1 关键词：四逆散；学习记忆； Q95-3 R285.5 A 1673-6273 2011 01-5-03 中图分类号： ， 文献标识码： 文章编号： （ ） Effects of Sinisan on Learning and Memory Expression of GABAB1 Receptor in the Brain of Fatigue Rats* LIU Yan, LIU Xiao-lan, SONG Yue-han, FAN Xin -liu, MA Jia-mei, WANG Ji, XU Zhan -j ia , LI Feng△ (Beij ing University of Traditional Chinese Medicine, Beij ing 100029) ABSTRACT Obj ective: To investigate the effects of Sinisan on learning and memory in fatigue model and the expression of GABA B1 receptor in rats brain. Methods: The Fatigue models were taken by the method of swimming traning and sleep deprivation. Evaluate learning and memory changes through Y maze and the changes of positive cell counts of GABA B1 receptors in rat brain by immunohis- tochemical method. Results: The correct response rate of Y-maze correctly were obviously lower in the model group than those in the normal control group. The error rate of Y maze were obviously higher in the model group than those in the normal control group. The expression of GABA