胰十二指肠切除术后肠黏膜屏障损伤与肠道细菌移位的临床(英文).pdfVIP

111 现代生物医学进展 Progress in Modern Biomedicine Vol.11 NO.1 JAN.2011 · · Clinical study on gut barrier dysfunction and bacterial translocation in patients after duodenopancreatectomy surgery* 1 2 2 1 1 SHAO Chang -chun , DING Lian -an , NIU Dong -guang , CHENG Hong -gang , CAO Shu -cheng 1 Qingdao University, Qingdao, Shandong 26600 1; 2 Dep artment of General Surgery, Affiliated Hosp ital of Medical College, Qingdao （ University, Qingdao, Shandong, China, 266003） ABSTRACT Obj ective: To investigate the relationship between bacterial translocation and acute systemic inflammatory state (SIRS) in patients who underwent duodenopancreatectomy surgery. Methods: 40 patients who underwent selective duodenopancreatectomy op- erations were observed. Blood were collected before surgery and 1, 3, 5d after surgery to detect plasma D-lactate and extract DNA.PCR analysis was performed with β-Galactosidase gene of Eschenchia coli and 16SrRNA gene as target gene. The SIRS of all the patients were observed for 10 days. Results: All the PCR results before operation were negative, while there was positive in 13 patients (32.5%, 13/40) after duodenopancreatectomy surgery. The positive PCR rate in SIRS was 85.7% (12/14), which was remarkably higher than that without SISR (3.8%, 1/26) (p0.01). 92.3% of the patient (12/13) with positive PCR result had SIRS while 7.4% patients (2/27) with neg- ative PCR result did have SIRS (p0.01). The plasma levels of D-lactate in patient with positive PCR result was significantly higher than those of the patients with negative PCR result (p0.01). The plasma levels of D-lactate in patient with SIRS was significantly h