正交试验优选春砂仁挥发油羟丙基-β-环糊精包合工艺.pdfVIP

正交试验优选春砂仁挥发油羟丙基-β-环糊精包合工艺Δ 1＊ 2 1 1# 张生潭 ，张 帆 ，汪铁山 ，林敬明 （1.南方医科大学珠江医院药剂科，广州市 510282；2.南方医科大学珠江 医院皮肤科，广州市 510282） 中图分类号 R284.2；R284.1 文献标识码 A 文章编号 1001-0408（2011）03-0225-04 摘 要 目的：研究羟丙基-β-环糊精（HP-β-CD）包合春砂仁挥发油的最佳工艺，并进行包合物鉴定。方法：以挥发油利用率、包合 物含油率与最高含油率比值和包合物收率的综合得分为评价指标，对搅拌法、超声法和研磨法进行优选；以挥发油与HP-β-CD 的 投料比、HP-β-CD 的浓度、包合时间、包合温度为考察因素，上述综合得分为评价指标，采用正交试验优选最佳包合工艺，并通过显 微鉴别、差示扫描量热分析、紫外分光光度、气相色谱质谱联用等方法对包合前、后的包合物进行鉴别。结果：最佳包合方法为超 声法，最佳包合工艺条件为挥发油与HP-β-CD 的投料比为1∶10（V ∶m ），HP-β-CD 的浓度为40％，包合时间80min ，包合温度 55℃。包合前、后成分未见明显变化。结论：春砂仁挥发油的HP-β-CD 包合工艺切实可行，可为实际生产研究提供一定的试验依据。 关键词 春砂仁；挥发油；羟丙基-β-环糊精；包合物；制备工艺 Optimization of the Preparation Technology of Amomum villous Oil-hydroxypropyl-β-Cyclodextrin Inclu- sion Complex by Orthogonal Experiment ZHANG Sheng-tan, WANG Tie-shan, LIN Jing-ming （Dept. of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China） ZHANG Fan （Dept. of Dermatology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China） ABSTRACT OBJECTIVE ：To optimize the preparation technology of Amomum villous oil-hydroxypropyl-β-cyclodextrin （HP-β-CD）inclusion complex, and to identify the inclusion complex. METHODS ：With the utilization ratio of volatile oil ，ratio of oil content to the highest oil content and yield of inclusion complex as index, the stirring method, ultrasonic method and trituration method were optimized. The preparation technology of inclusion complex was optimized by orthogonal experiment taking the com- prehensive evaluation score as index with ratio of volatile oil to HP-β-CD, concentration of HP-β-CD, inclusion time and inclusion temperature as factors. Microscopic identification method, DSC method ，UV method and GC-MS method were used for the identifi- cation of inclusion co