卵巢激素撤除you发雌性小鼠抑郁样状态(英文).pdfVIP

·56 · 现代生物医学进展 Progress in Modern Biomedicine Vol.11 NO.1 JAN.2011 Depressive-like effect of ovarian hormone withdrawal in female mice 1△ 1 1 2 2 REN Li-xiang , LUO Yi-fan , LEI Jiang , ZUO Dai-ying , WU Ying -liang 1 Shenyang JHealth Bio-technic Development Co., Ltd, Shenyang 1100 16, China; （ 2 Schoole of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang 1100 16, China） ABSTRACT Obj ective: To investigate the depressive-like effect of ovarian hormone withdrawal on female mice and to investigate the possible neurochemistry mechanism. Methods: The ovaries of female mice at 9 weeks were bilaterally resected. The day after the surgery, the mice were treated by diaethylstilbestrol (DES) or fluoxetine (FLX) once per day for 2 weeks. Then the forced swimming test (FST) and tail suspension test (TST) were detected. The norepinephrine (NE), dopamine (DA) ,5-hydroxytryptamine (5-HT) in the hypothalamus and the hippocampus were detected by high-performance liquid chromatography with an electrochemical detector (HPLC-ECD). Results: The ovariectomy increased significantly at the duration of immobility in both FST and TST. The ovariectomy decresed significantly the NE and DA level in the hypothalamus, also produced a marked decline of NE and 5-HT in the hippocampus. Chronic treatment by DES or FLX improved the impairment of behavioral performance and attenuated the decline of monoamines induced by ovariectomy. Conclusion: The bilateral ovariectomy induces depressive-like state in female mice, which may be analogous to some aspects of depression in menopause.The neurochemistry mech