输尿管部分梗阻论文：血清CystatinC、β2-MG、UA在兔单侧输尿管部分梗阻性肾病中的意义.docVIP

输尿管部分梗阻论文：血清CystatinC、β2-MG、UA在兔单侧输尿管部分梗阻性肾病中的意义 【中文摘要】:利用兔单侧输尿管部分梗阻模型（PUUO），探讨梗阻后不同时期联合测定血清Cys C、β2-MG、UA在肾积水肾功能损害的诊断、治疗和恢复中的应用价值。同时观察梗阻后不同时期肾组织的病理特点，为临床上及时使用干预肾脏纤维化的药物及手术解除梗阻的时机提供实验依据。方法：健康新西兰雄性大白兔50只，对照组10只，对照组只进行输尿管游离；实验组按术后1周、2周、4周、8周分为4组，每组10只，用输尿管套管法建立PUUO模型。按实验设定的不同时间段检测兔血清Cys C、β2-MG、UA。以核素肾动态显像测得的肾小球滤过滤（GFR）为标准，根据上述检测结果比较Cys C、β2-MG、UA与GFR的相关性。各组大白兔在完成核素肾动态显像两天后处死，取其肾脏，光镜观察病理变化。结果：①随着梗阻时间的延长，各项检测指标逐渐增加，PUUO模型的兔血清CysC、β2-MG、UA在输尿管部分梗阻后1周与对照组相比差异无统计学意义（P＞0.05），梗阻后2周、4周、8周不同时间点均显著高于对照组（P0.01）。②血清Cys C、β2-MG、UA与GFR存在着显著相关关系，且均呈负相关。③肾脏病理检测显示：在梗阻早期，即梗阻第1周，肾小管轻度扩张，肾间质水肿不明显，间质内少量炎症细胞侵润，以单核细胞、淋巴细胞为主；随着梗阻时间的延长，上述表现逐渐加重，肾小管进一步扩张;至第8周时，炎性细胞减少，间质内代之以成纤维细胞为主，最终发展为肾小球硬化及肾间质纤维化。结论：①血清Cys C、β2-MG、UA是反映GFR较理想的血清标志物，对肾积水肾功能损害程度的判断具有极其重要的意义；②动态观察上述指标可用于指导临床上肾积水肾功能损害程度的诊断、治疗和恢复情况；③肾脏病理学检查能及时发现肾纤维化，为及时使用干预肾脏纤维化的药物和手术解除梗阻的时机提供依据。 【英文摘要】: To use some rabbits with partial unilateral ureteral obstruction model is toexplore the value of CystatinC、β2-microglobulin、Uric Acid in different stages fordiagnosing，recoverying and treating renal dysfunction. The pathological changes ofkidney testis were observed after different stages, which is to guide clinic therapy withintervene kidney fibrosis.Methods:Fifty health male rabbits were divided into a control group and aexperimental group. The rabbits in control group (n=10)were only made ureter free.The experimental group, after 1week, 2weeks, 4weeks, 8weeks were devided into 4groups(n=10) were made partial unilateral ureteral obstruction model. CystatinC，β2-microglobulin，Uric Acid were measured in rabbits of partial unilateral ureteralobstruction. Glomerulaf filtration rate(GFR)by radionuclide renography wasconsidered to be a standard to decide the correlation between GFR and the above threeparameters. Rabbits in each group , were completed nuclide renal dynamic imagingafter two days, were put to death, and take the kidney; The pathological changes ofkidney testis were observed by light microscope.Results:①With the extension of time of obst