妊娠期糖尿病论文：HMGB1、RAGE及TNF-α在妊娠期糖尿病胎盘组织中的表达及相关性研究.docVIP

妊娠期糖尿病论文：HMGB1、RAGE及TNF-α在妊娠期糖尿病胎盘组织中的表达及相关性研究 【中文摘要】观察妊娠期糖尿病患者及正常孕妇胎盘组织中HMGB1、RAGE及TNF-α的蛋白表达及其相互作用与妊娠期糖尿病的关系。探讨三者在妊娠期糖尿病发病机制中的作用及相关性,为该病的治疗及预防开拓新的思路。方法：收集广西医科大学第一附属医院产科2011年7月至2012年2月收治的30例妊娠期糖尿病患者(实验组),及同期住院的正常孕妇(正常妊娠组)31例,采用免疫组化的方法检测妊娠期糖尿病患者(实验组)和对照组正常孕妇胎盘组织中HMGB1、RAGE及TNF-α蛋白的表达。结果：(1)两组胎盘组织均有HMGB1、RAGE及TNF-α蛋白的表达；(2)妊娠期糖尿病组胎盘组织中HMGB1、RAGE和TNF-α蛋白的表达水平均高于正常对照组,差异有统计学意义(p0.05)；(3)GDM组胎盘组织中HMGB1、RAGE(?) TNF-α三者之间的关系呈正相关(r=0.876,p0.01r=0.784,p0.01 r=0.853,p0.01)。结论：妊娠期糖尿病组胎盘组织中HMGB1、RAGE及TNF-α表达较对照组增高提示三者可能参与了妊娠期糖尿病患者机体慢性炎症反应,从而引起胰岛素抵抗和胰岛细胞损伤等糖尿病病理生理过程,其中TNF-α可能通过刺激巨噬细胞活化调节HMGB1的表达,而HMGB1与其受体RAGE结合后又可刺激单核巨噬细胞释放TNF-α,形成正反馈,两者相互及协同作用维持和放大炎症反应,从而参与妊娠期糖尿病的病理生理改变。 【英文摘要】Object:The purpose of this study was to investigate the expression and correlation of HMGB1、RAGE、TNF-a in gestational diabetes mellitus. we measured the level of HMGB1、RAGE、TNF-αprotein expression in placenta tissues obtained from the patient with gestational diabetes mellitus and normal pregnancy, to explore the difference and relationship between them and provide a new thinking way for clinical treatment and prevention.Method:A case-control study was performed in 30 women with gestational diabetes mellitus and 31 cases of normal 50g glucose challenge test(50g GCT)as control recruited from Jul 2011 to Tue 2012 in department of Obstetrics, the First Affiliated Hospital of Guangxi Medical University. All the samples were gotten from the maternal surface of the placenta after the labor immediately. To compare their clinical feature and the localized protein expression of HMGB1、RAGE、TNF-αin placenta of gestational diabetes mellitus and normal pregnancy by immunohistochemistry way.Result:(1) Women of two groups had expressed HMGB1、RAGE and TNF-a;(2)The localized protein expressions of HMGB1、RAGE and TNF-a were significantly higher in placenta of gestational diabetes mellitus than in control group (p0.01,p0.05 respectively). (3) There showed a significant linear positively correlation among HMGB1、RAGE and TN