猫抓病论文：汉赛巴尔通体体外红细胞感染实验.docVIP

猫抓病论文：汉赛巴尔通体体外红细胞感染实验 【中文摘要】巴尔通体(Bartonella)是一群革兰氏染色阴性、氧化酶阴性、营养条件要求苛刻兼性细胞内寄生的需氧杆菌,从20世纪80年代开始再次在全球范围内流行感染,随着检测技术的发展,巴尔通体感染引起的临床疾病日益增多。然而学术界任然将该病视为一种被忽视的人兽共患病。其中汉赛巴尔通体(Bartonella henselae)是巴尔通体属中重要的致病菌之一,直到20世纪90年代才被发现,由于其对动物和人的广泛致病性和复杂的临床特征,已引起国内外众多学者的研究兴趣。汉赛巴尔通体是一种革兰氏阴性棒状短小杆菌,是临床上引起人类发生猫抓病(cat-scratch disease, CSD)的常见病原。猫是汉赛巴尔通体主要宿主,该病原通过猫蚤在猫和猫之间传播,被猫抓伤或咬伤则可导致该病原从猫传播到人。体外感染模型构建对于病原致病机理研究具有重要意义。目前关于汉赛巴尔通体体外感染模型研究尚未得出一致结果,本论文拟开展体外红细胞感染模型构建研究,通过庆大霉素保护试验及透射电镜观察汉赛巴尔通体体外红细胞感染效率,以期为后期致病机理研究奠定基础。 【英文摘要】Bartonella spp. is a small, fastidious, intracellular Gram-negative bacteria that has been identified recently in a wide range of domestic and wild mammals. The genus Bartonella has expanded in the recent 15 years from the single Bartonella bacilliformis to at least 20 recognized species and subspecies, in which at least 10 of these species and subspecies are known or suspected to be pathogenic for humans. As the improvement on its diagnosis continues, knowledge of the spectrum of clinical diseases resulting from Bartonella infection is expanding. Three Bartonella species, B. bacilliformis (Carrion抯 disease), B. henselae (cat-scratch disease), and B. quintana (trench fever), have been well elucidated as primary pathogens of human bartonellosis. Of these, Bartonella hensalea is the most common pathogen induced human bartonellosis. Establishment of infection model in vitro is important for study of the mechanism of host-pathogen interaction. However, in vitro studies on the intra-erythrocytic parasitism of bartonella hensalea had not been confirmed with different results obtained from different research groups. Here, we aim to establish the in vitro model of Bartonella hensalea by using gentamycin protection assay and transmission electronic microscope. 【关键词】猫抓病 汉赛巴尔通体 体外感染 【英文关键词】cat-scratch disease Bartonella henselae Vitro infection 【目录】汉赛巴尔通体体外红细胞感染实验 摘要 6-7 Abstract 7-8 符号说明 11-12 第一章 文献综述 12-22 1 巴尔通体生物学特性 12-14 1.1 汉赛巴尔通体形态特征 12-13 1.2 汉赛巴尔通体生理生化特征 13 1.3 汉