不同DO下MBR内微生物群落结构与运行效果关系_高大文不同DO下MBR内微生物群落结构与运行效果关系_高大文.pdf

中国环境科学 2010,30(2) ：209~215 China Environmental Science 不同 下 内微生物群落结构与运行效果关系 DO MBR * 高大文,李昕芯,安 瑞,付 源,任南琪 (哈尔滨工业大学城市水资源与水环境国家重点实验室,黑龙江 哈尔 滨 150090) 摘要：应用A/O-MBR 处理实际生活污水,考察了不同溶解氧(DO)条件下,微生物群落结构与其处理效果的对应关系.结果表明,DO 浓度在 对 去除效果无明显影响 平均去除率均在 以上 浓度变化对 +- 去除影响较大 浓度下降到 0.2~4.0mg/L COD ,COD 90% .DO NH4 N ,DO 0.2mg/L 时,NH4+-N 平均去除率由99%下降到65%.通过PCR-DGGE 分析,较高DO 条件下(4.0,2.0mg/L)的总细菌微生物群落多样性高于较低DO 条件(0.5,0.2mg/L),但其群落结构变化与与反应器的处理效果对应关系不明显;氨氧化菌的群落结构变化较明显,且在不同的 DO 条件下起 主要作用的氨氧化菌的菌属不同,其群落变化与反应器的NH4+-N 去除效果相对应,DO 为2.0,0.5mg/L 时氨氧化菌群落结构比较相似,此时 反应系统的脱氮效果也比较好. 关键词：膜生物反应器；溶解氧； - ；微生物群落结构；氨氧化菌 PCR DGGE 中图分类号：X703.1 文献标识码：A 文章编号：1000-6923(2010)02-0209-07 Relationships between microbial community structure and the performance of MBR under different dissolved * oxygen. GAO Da-wen , LI Xin-xin, AN Rui, FU Yuan, REN Nan-qi (1.State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China). China Environmental Science, 2010,30(2) ：209~215 Abstract ：Microbial community structure and nutrient removal performance under different dissolved oxygen were investigated using Anoxic-oxic submerged membrane bioreactor (A/O-MBR) for municipal wastewater treatment. Results showed that the COD removal was not influenced markedly by the variations in DO between 0.2mg/L and 4.0mg/L, and the COD removal was more than 90% during the whole experiment. However, the ammonia nitrogen removal was more sensitive than COD, and the ammonia nitrogen removal dropped from 99% to 65% when DO decreased to 0.2mg/L. The PCR-DGGE analysis showed tha