日本恶性血液病分子遗传学的发展（英文）.ppt

K562 K562/ WT K562/ TM K562/ CD-1 K562/ CD-2 K562/ CD-3 CD20 expression in K562 cells with C-terminal deleteion Surface CD20 Western blot analysis using N-terminal antibody for CD20 antigen 　　　　　　　　 normal C-terminal deletion group CR #(%) 19 (49) 1 (25) Non-CR #(%) 21 (51) 3 (75) 　 計 40 4 Response to R-containing treatment in both normal and CD20 mutated cases Progression-free survival after R-containing chemo in normal and C-terminal deletion group n=4 n=40 Conclusion 　　C-terminal deletion mutation is related to loss of CD20 and shortened PFS Other MoAbs B-cell lymphoma CD20 Rituximab、GA101、HumaxCD20 Zevalin… CD22　　CMC-544… T-cell lymphoma CD4 HumaxCD4 CCR4 Anti-CCR4 Ab B-cell ：82.7 ％ T/NK-cell ：10.3 ％ HL ：7.0 ％ B-cell lymphoma n=414 T/NK-cell lymphoma n=53 Overall survival rate CHOP n=24 : 58% Other therapy n=10 : 57% Overall survival rate PTCL Clinical aspects of T-cell lymphoam in CIH B-cell lymphoma T/K lymphoma IGF1R Receptor tyrosine kinase １５q２６ chromosome １３６７A.A., single polypeptide 　　cleaved by protease and α subunit and β subunit makes heterodimer and expressed on the surface 　　 β chain is degraded by ubiquitin/proteasome　 Expressed on T cells（CD４＞CD８） Homology with IR, ~７０％ IGF1R expression on cell lines Jurkat TALL CCRF KMS-BM KMS-PE IM-9 IM-9 VR IGF1Rα IGF1Rβ β-actin Jurkat K562 Raji Daudi KYSE BALL-1 ARH SKW IGF1R expression on the cell surface IGF1Rα IGF1Rβ β-actin The effect of Glucose on Jurkat cell proliferation With bortezomib Hours in culture 0.1% Glucose 2.0% Glucose x 104 cells/ml The effect of glucose for IGF1R expression changes on Jurkat cells 0.1% Glucose 2.0% Glucose 0 1 2 3 4 0 1 2 3 4 IGF1Rα IGF1Rβ β-actin Day The effect of bortezomib on Jurkat cells Bortezomib (10nM) Hours in culture x 104 cells/ml 0.1% Glucose 2.0% Glucose IGF1R expression