吸附-包埋结合法共固定化糖化酶和酵母菌的研究-河南农业科学.PDF

※生物工程 食品科学 2009, Vol. 30, No. 05 201 侯红萍，闫跃文 ( 山西农业大学食品科学与工程学院，山西太谷 030801) ：以聚乙烯醇和海藻酸钠为包埋材料，以硅藻土或活性炭或Al2 O3 为吸附剂，采用吸附- 包埋结合法共固 定化糖化酶和酵母菌。实验结果表明，聚乙烯醇和海藻酸钠的最佳配比是9:1 ，最适吸附剂为硅藻土，其添加 量为0.6g/20ml ；实验还研究了共固定化颗粒的发酵性能。结果显示，其最佳发酵条件是底物浓度25% 、发酵温 度30 ℃、起始pH5.0 、糖化发酵时间7d ，此条件下发酵酒度达11%(V/V) 。 ：聚乙烯醇；海藻酸钠；共固定化；吸附- 包埋法；硅藻土 Co-immobilization of Glucoamylase and Yeast by Adsorption-embedment Method HOU Hong-ping ，YAN Yue-wen (College of Food Science and Engineering, Shanxi Agricultural University, Taigu 030801, China) Abstract ：Glucoamylase and yeast were co-immobilized by adsorption-embedment method with polyvinyl alcohol (PVA)- sodium alginate as embedding materials and with diatomite or activated carbon or alumina as adsorbent. The results showed that the optimum ratio of PVA to sodium alginate is 9:1 and the best adsorbent is diatomite at the addition level of 0.6 g/20 ml. Moreover, the experimental results on the saccharification and fermentation performance of co-immobilized beads indicated that the optimum sorghum powder concentration, fermentation temperature, initial pH value and saccharification-fermentation time are 25%, 30 ℃, 5.0 and 7 d, respectively. Under the above conditions, the alcohol content is up to 11% (V/V). After the co- immobilized beads are continually used for 10 batches, their performances are still stable. Key words ：polyvinyl alcohol (PVA) ；sodium alginate；co-immobilization ；adsorption-embedment method ；diatomite 中图分类号：Q814 文献标识码：A 文章编号：1002-6630(2009)05-0201-04 随着固定化技术的发展，多种微生物、多种酶以 产的方向发展[4] 。本研究以聚乙烯醇(PVA)和海藻酸钠 及酶与微生物的共固定化已成为现代酶工程技术的研究 为包埋材料