巫山淫羊藿悬浮培养细胞的生长特性研究-天津中草药杂志社.PDF

中草药 Chinese Traditional and Herbal Drugs 第 42 卷 第 8 期 2011 年 8 月 • 1609 • 巫山淫羊藿悬浮培养细胞的生长特性研究 1 2* 韩素菊 ， 黎云祥 1. 绵阳师范学院，四川 绵阳 621000 2. 西华师范大学 西南野生动植物资源保护教育部重点实验室，四川 南充 637002 摘 要：目的 考察巫山淫羊藿悬浮培养细胞的生长特性，为巫山淫羊藿资源利用提供参考。方法 选用悬浮培养所获得 的均匀悬浮培养细胞为研究对象，在光照条件下进行悬浮培养，每隔 3 d 取样，测定相关的生理生化指标。结果 巫山淫 羊藿悬浮细胞的生长曲线呈“S”型，培养基中蔗糖、磷酸盐、硝酸盐、铵盐量均呈急剧下降趋势，最后变化趋于平缓， 细胞合成黄酮最佳光照时间为每天 12 h，黄酮合成量为 27.854 82 mg/L 。结论 巫山淫羊藿细胞生长特性的研究为其大规 模悬浮培养生产天然活性成分奠定了基础。 关键词：巫山淫羊藿；悬浮培养；生长曲线；生长特性；黄酮 中图分类号：R282.21 文献标志码：A 文章编号：0253 - 2670(2011)08 - 1609 - 05 Growth characteristics of Epimediun wushanense in cell suspension culture 1 2 HAN Su-ju , LI Yun-xiang 1. Mianyang Normal University, Mianyang 621000, China 2. Key Laboratory of Southwest China Wildlife Resources Conservation, Ministry of Education, China West Normal University, Nanchong 637002, China Abstract: Objective To provide biotechnology reference for the utilization of Epimedium wushanense. Methods Some subculture conditions were determined and the growth characteristics of suspension cells were observed every 3 d. Results The cell showed S-shaped growth curve and the content of nitrate, ammonia salt, phosphate, and sucrose all showed sharply decreasing trend during the initial cell suspension culture of E. wushanense. Then the respective changes slowed down. The optimum illumination duration was 12 h. The highest output of flavonoids was 27.854 82 mg/L under 12 h illumination. Conclusion The growth characteristics research lays the foundation of mass natural active ingredient in cell suspension culture production of E. wushanense . Key words: Epimediun wushanense T. S. Ying; suspension culture; growth curve; growth characteristics; flavonoids 巫山淫羊藿Epimediun wushanense T. S. Ying 是 代谢产物具有提高产率、缩短周期、调控其生产过 小檗科淫羊藿属的多年生宿根性草本植物。《本草纲