杜仲叶提取绿原酸的中间试验研究-林产化学与工业.PDF

23 3 Vol. 23 No. 3 2003 9 Chemistry and Industry of Forest Products Sept. 2003 马希汉, 尉 芹, 景谦平 (西北农林科技大学生命科学学院, 陕西 杨陵712100) MA X H : , : 40% ( 80 ,2 h, 116 , 2 ) ( ,80 ) (4 ) ( 100 g 140 g ) (20% H SO ) ( 20 ) 2 4 ,0.53%, 98.35% : ; :Q949.751.5; R284.2 :A :0253- 2417(2003) 03- 0073- 04 STUD ON TECHNIQUE UPON ISOLATION AND PURIFICATION OF CHLOROGENIC ACID FROM LEAVES OF EUCOMMIA MA X-i han, WEI Qin, JING Qian-ping ( College of Lfi e Sciences, N orthwest Sci-Tech University of A griculture and Forestry , Yangling 712100, China) Abstract: Based on the previous study,chlorogenic acid was isolated and purified from leaves of ucommia ulmoides Oliver in an pilot-scale experiment by thefollowing process: refluxing by 40% ethanol(80 ,2 h,ratio of material to solvent: 116, extract twice) extracts preparation( vaccum evaperation, drying at 80 ) extraction by water ( 4 times)precipitation by Pb(OAc) [ 140 g Pb(OAc) : 100 g leaves] resolution of chlorogenic acid by 20% sul- 2 2 furic acid extraction by ethyl acetate( 20 times) condensationre-crystallization. The yield of chlorogenic acid is 0.53%, at purity 98. 35%. Results of the experiment showed that this technological process was feasible and practical. It was found that no isomers of chlorogenic acid occurs in the leaves of . ulmoides after comparing with honeysuckleflowers and raw coffee beans, in which the occurrence of iso-chlorogenic acids was reported. This is traceable t