沼泽红假单胞菌胞内番茄红素生物合成的研究-河南农业科学.PDF

※生物工程 食品科学 2013, Vol.34, No.21 283 沼泽红假单胞菌胞内番茄红素生物合成的研究 1 2 1,* 1 张 同，朱瑞艳，惠伯棣 ，杨博媛 (1.北京联合大学应用文理学院，北京 100 191；2.河北省生物新能源工程技术研究中心，河北 秦皇岛 066004) 摘  要：研究沼泽红假单胞菌中番茄红素的合成积累，为开发番茄红素的新资源作出尝试。通过HPLC和HPLC-MS 3,4- 分析沼泽红假单胞菌四氢呋喃萃取液，确认萃取液中所含的主要类胡萝卜素为玫红品、 脱氢玫红品和番茄红 素。在补充碳源的条件下，以种子培养基培养沼泽红假单胞菌，确定番茄红素在菌群进入对数生长期一段时间后才 大量积累，并在菌群因缺少氮源而进入稳定期后开始下降。这说明番茄红素的合成不仅需要碳源作为前体物，还需 要足够的氮源生成类胡萝卜素合成酶才可以大量积累。通过实验分别确定了沼泽红假单胞菌生产番茄红素的最佳碳 pH 0.2456μg/mL 0.1682μg/g 源、氮源及 值，在最佳条件下番茄红素的总产量最大值为 ，相对产量为 。 关键词：番茄红素；沼泽红假单胞菌；生物合成；培养优化 Lycopene Biosynthesis in Rhodopseudomonas palustris ZHANG Tong1，ZHU Rui-yan2 ，HUI Bo-di1,*，YANG Bo-yuan1 (1. College of Applied Arts and Science, Beijing Union University, Beijing 100191, China ； 2. Hebei New Bio-energy Engineering Research Center, Qinhuangdao 066004, China) Abstract ：This research aims to study the biosynthesis of lycopene in Rhodopseudomonas palustris and explore the possibility for Rhodopseudomonas palustris to be a novel source of natural lycopene. The metabolic products of R. palustris extracted with tetrahydrofuran (THF) were analyzed by HPLC and HPLC-MS. The results showed that the main carotenoids in R. palustris were rhodopin, 3,4-dehydrorhodopin and lycopene. R. palustris was cultivated in SMN supplemented with carbon source. Massive lycopene accumulation was observed during a period after the beginning of the logarithmic phase, followed by a decline at the stable phase. The optimal carbon source, nitrogen source and pH were dete