医学论文之药物学科论文汇编.doc

医学论文之药物学科论文汇编 目 录 穿心莲内酯对不同分化胃癌细胞株的体外增殖的影响 1 黄芪雷公藤合剂对链脲菌素诱导的糖尿病大鼠肾损伤的防治效果 4 清毒片、养正片对白血病细胞uPA活性影响的研究 6 三黄软膏治疗急性软组织损伤的实验研究 9 166例药品不良反应报告分析 10 黄连不同炮制品的体外抑菌实验研究 11 绵毛鹿茸草的次生代谢产物含量与抑菌活性分析 13 木香类药材鉴别要点 16 鹿茸多肽对β淀粉样蛋白诱导脊髓神经元细胞凋亡的保护作用 18 仙鹿健骨汤含药血清对成骨细胞增殖和ALP影响的实验研究 24 白及甘露聚糖抗胃溃疡及抗炎、镇痛作用的实验研究 26 老年患者的中成药合理应用 29 五味子提取物体外抑菌作用初探 29 医院药学管理体会 31 药品研发项目的风险识别研究 32 机械化学法辅助提取刺五加总黄酮的工艺研究 34 超声波提取鱼腥草多糖工艺研究 39 穿心莲内酯对不同分化胃癌细胞株的体外增殖的影响 作者：董建华 叶再元 李曙光 陶厚权 杨琼 【摘要】? ［目的］明确穿心莲内酯对不同分化胃癌细胞株的体外增殖的影响。［方法］以不同浓度的穿心莲内酯处理低分化(MKN45)、中分化 (SGC7901)和高分化（MKN28）胃腺癌细胞株，分别温育24h 、48h 、72h；采用噻唑蓝比色法检测各和穿心莲内酯对癌细胞的药物半数抑制浓度(IC50 )，比较穿心莲内酯对不同分化胃癌细胞株的体外增殖抑制作用的异同。［结果］穿心莲内酯对胃癌细胞株MKN45、SGC7901、MKN28的体外增殖均具有显著的抑制作用，并且呈剂量效应和时间效应关系。低分化的MKN45细胞株对穿心莲内酯的敏感性明显高于高分化的MKN28细胞株和中分化的SGC7901细胞株。［结论］穿心莲内酯对不同分化程度的胃腺癌细胞株体外增殖抑制作用不同。 【关键词】? 穿心莲内酯；分化程度；胃癌细胞株；敏感性；MTT法 　　Abstract：［Objective］ To determine the sensitivity of Andrographolide in gastric cancer cell lines in various degrees of differentiation.［Methods］ Three gastric cancer cell lines including MKN45(1ow degree differentiation)，SGC7901(moderate degree differentiation) and MKN28 (high degree differentiation) were incubated with Andrographolide of various concentration at 24 hours，48 hours，72 hours.The optial density （OD）value of each group was examined by MTT test；compute? the data to arrive at inhibition ratio（IR）and 50% inhibiting concentration ( IC50 )，make the sensitivity disparity clear by compare? the data.［Results］ Andrographolide had significant inhibition effects on the proliferation of MKN45，SGC7901 and MKN28 cells in vitro,in a time—dependent and dose—dependent way.Poorly differentiated gastric cancer cell line MKN45 is more sensitivity to chemotherapy than well differentiated MKN28 and moderately differentiated SGC7901.［Conclusion］ The effect of Andrographolide on gastric cancer cells in various degrees of differentiation were different. 　　Key words:Andrographolide； degrees of differentiation；gastric cancer cell1ines；sensitivity；MTT method??? 　　穿心莲内酯（Androgra