枯草芽孢杆菌对肉鸡生长性能及其抗氧化和免疫功能的影响.docVIP

枯草芽孢杆菌对肉鸡生长性能及其抗氧化和免疫功能的影响 毛翔飞 秦 艳 李卫芬* (浙江大学动物科学学院，农业部重点开放实验室，浙江杭州 310029) ? 摘 要：本试验旨在研究枯草芽孢杆菌(B.subtilis)对罗斯308肉鸡抗氧化能力和免疫性能的影响。试验选用1日龄罗斯308肉鸡216羽，随机分为2组，每组设3个重复。对照组饲喂基础日粮，处理组在基础日粮中添加B.subtilis 105 CFU/g。饲养试验期为42 d。结果表明：与对照组相比，处理组肉鸡日增重提高了12.20%(P0.05)，血清与肝脏的总抗氧化能力(P0.05)以及谷胱甘肽过氧化物酶活性(P0.01)显著增加；血清中丙二醛(MDA)、NO及肝脏中MDA水平(P0.05)显著降低。此外，日粮中添加B.subtilis显著提高了肉鸡胸腺指数、法氏囊指数和血清中IgG水平(P0.05)，而对脾脏指数及血清中溶菌酶、白介素-2、肿瘤坏死因子-α等指标无显著影响(P0.05)。结果提示，日粮中添加适量B.subtilis可通过提高肉鸡的抗氧化与免疫功能而改善其生长性能。 关键词：枯草芽孢杆菌；抗氧化力；免疫性能；肉鸡 Effects of B.subtilis on Growth Performance，Antioxidant and Immunity of Broilers YU Dong-you，MAO Xiang-fei，QIN Yan，LI Wei-fen* (Key Laboratory of Ministry of Agriculture，Zhejiang University，Zhejiang Hangzhou 310029，China) Abstract：This study was conducted to examine the effects of B.subtills on growth performance，antioxidant and immunity of broilers．A total of 216 day-old Ross 308 broilers were divided into the control group (basal diet) and the treatment group (basal diet supplemented with l05 CFU/g B.subtilis)．Each group had three replicates and each replicate included 36 broilers (half male and half female)．The experiment lasted for 42 d．The results showed that ADG，T-AOC and the activity of GSH-Px of the treatment group were improved significantly compared to the control group，while the level of serum MDA，NO and MDA in liver were decreased significantly．The B.subtilis could improve thymus index，bursa index and the level of serum IgG，but did not affect spleen index，serum lysozyme，IL-2 and TNF-α significantly．These results indicated that growth performance of broilers fed diet with B.subtilis was improved by increase of antioxidant and immunity． Key words：B.subtile；antioxidant；immunity；broiler chickens ? 益生菌如芽孢杆菌、乳酸菌等以其独特的作用方式和生态效应，已被广泛应用于食品、医疗、畜禽和水产养殖等领域。枯草芽孢杆菌(B.subtilis)可产生70多种抗菌素及多种酶类，是目前应用较广泛的芽孢杆菌。已有研究表明，芽孢杆菌在不利环境条件下能够以孢子形式存在，在饲料制粒、贮存及胃酸环境中仍能保持较高的活性，在改善畜禽生产性能、维持肠道菌群平衡、提高消化率等方面均有较好的效果，目前的研究也主要集中在这几方面[1，2]。但有关芽孢杆菌对动物抗氧化和免疫功能影响的报道较少。为此，本试验以罗斯308肉鸡为试验对象，研究B.subtilis对肉