提取类毕业论文.doc

攀枝花学院本科毕业设计（论文） 金龙胆草中总皂苷的提取研究 学生姓名： 学生学号： 院（系）： 年级专业： 指导教师： 二〇一二年五月  摘要 本实验采用水浴回流提取，分别通过单因素实验、正交实验考察金龙胆草总皂苷的最佳工艺条件，为金龙胆草总皂苷药理药效研究提供材料，同时为其进一步开放奠定基础。方法 以熊果酸为对照品，用紫外可见分光光度法建立熊果酸的标准曲线；在单因素试验的基础上，以总皂苷含量为指标，采用正交试验方法考察提取温度、固液比、乙醇浓度、提取时间等因素对提取工艺参数做初步研究。结果　（1）单因素实验结果：设计金龙胆草的提取工艺为乙醇浓度分别为50%，60%，70%，80%，90%；固液比分别为1：20，1：25，1:30，1：35，1：40；提取温度分别为50℃，60℃，70℃，80℃，90℃；提取时间分别为0.5h，1h，1.5h，2h，2.5h；（2）正交实验结果：提取温度A，固液比B，乙醇浓度C对指标影响显著。根据以上分析，可定出影响指标的因素显著性主次顺序为：ABCD。总皂苷的优选浸提条件（各因子优水平组合）为最佳提取温度70℃，，最佳固液比1:30，最佳乙醇浓度为80%，最佳提取时间2h。 关键词：金龙胆草； 总皂苷；水浴回流提取； 正交试验法  ABSTRACT The purpose of Water bath reflux method extraction process of total Saponins in Gold gentian conditions of a preliminary study and with the traditional craft make that nol compared to optimize the extraction of total Saponins in Gold gentian conditions. Method Use Ursolic acid as reference substance, by ultraviolet-visible spectro photometry Ursolic acid standard curves set up; in the single factor test, based on the total Saponins content as an index, using orthogonal test approach to Extraction temperature solid- liquid ratio, ethanol concentration , extraction time on the extraction process parameters to do a preliminary study. Conclusion The gentian design gold extraction process is the concentration of ethanol were 50%, 60%, 70%, 80%, 90%; solid-liquid ratio were 1:20,1:25,1:30,1:35,1: 40; extraction temperature is 50 ° C, 60 ° C, 70 ° C, 80 ° C, 90 ° C; extraction time for 0.5h, 1h, 1.5h, 2h, 2.5h; The experimental results show that: The yield of total Saponins as an index, Water bath reflux method extraction is superior to the traditional reflux extraction method, total Saponins in Gold gentian the best extraction process for the 80% ethanol concentration, solid-liquid ratio of 1:30, Extraction temperature to 70Degrees Celsius, extraction time to 2hour. Key words: Gold gentian, total Saponins, Water bath reflux me