淡水鱼糜漂洗水中水溶性蛋白质回收的研究.docx

淡水鱼糜漂洗水中水溶性蛋白质回收的研究吴正奇1，李佳文1，石勇1，袁江兰1，何秋生2，黎鸣放2，李向阳21.湖北工业大学生物工程学院 (武汉 430068)；2.武汉梁子湖水产品加工有限公司 (武汉 430000)研究和比较了四种不同回收剂回收淡水鱼糜漂洗水中水溶性蛋白质的特性。首先，通摘要过正交试验法以蛋白质的回收率为指标，优化三氯化铁、阳离子聚丙烯酰胺、明矾和硫酸锌回收淡水鱼糜漂洗水中水溶性蛋白质的的最佳条件，分别为三氯化铁浓度0.05%、pH值6.0、温度20℃和时间50 min；阳离子聚丙烯酰胺pH值3.0，浓度0.4%，时间70 min和温度20℃；明矾浓度0.2%，pH6.0，温度20℃和时间50 min以及硫酸锌时间70 min，温度15℃，浓度0.2%和pH7.5。其 次，测定四种不同回收剂在最佳条件下回收淡水鱼糜漂洗水中水溶性蛋白质的回收率，最大回 收率分别为三氯化铁83.26%、阳离子聚丙烯酰胺70.78%、明矾74%和硫酸锌61.56%。从回收效 果、卫生安全性和成本角度综合考虑，三氯化铁法和硫酸锌法具有实际可应用性。关键词 淡水鱼糜漂洗水；水溶性蛋白质；回收；三氯化铁Study on the Recovery of Water Soluble Protein from the WashingWater of Freshwater Fish SurimiWu Zheng-qi1，Li Jia-wen1，Shi Yong1，Yuan Jiang-lan1，He Qiu-sheng2，Li Ming-fang2，Li Xiang-yang21. Bioengineering College of Hubei University of Technology ( Wuhan 430068);2.Wuhan Liangzihu Aquatic Products Processing Ltd.Company ( Wuhan 430000)Abstract The characteristics of four different substances using for the recovery of water soluble protein(WSP) from the washing water of freshwater fish surimi(WWFFS) were studied in this paper. Firstly, their optimum conditions for the recovery of WSP from WWFFS were optimized through fourthe orthogonal tests of L (34) using the protein recovery ratio as decided index, results showed that the9four optimum conditions were concentration 0.05%, pH 6.0,temperature 20℃ and time 50 min forFeCl , concentration 0.4%, pH 3.0, temperature 20℃ and time 70 min for polyacrylamide(CPAM),3concentration 0.2%, pH 6.0, temperature 20℃ and time 50 min for Alums, and concentration 0.2%,pH 7.5,temperature 15℃ and time 70 min for ZnSo , respectively. Secondly, their recovery ratios in4their optimum conditions were analyzed as 83.26% for FeCl , 70.78% for CPAM, 74% for Alums and356% for ZnSo . FeCl and ZnSo can practicably use for recovery WSP from WWFFS comprehensively4 34compared recovery ratio, safety and cost.Keywords washing water of freshwater fish surimi；water soluble protein；recovery；FeCl3漂洗是除去淡水鱼鱼肉中的色素、降低鱼糜弹性因子、水溶性成分、脂肪和腥臭味物质以提高鱼糜 质量的最重要工序。鱼糜漂洗水的主要成分虽然