短时受精方式对受精结果的影响-第三军医大学学报.doc

短时受精对体外受精结果的影响 屈宗银，王凤英，李玉艳，何畏，俞炽阳，李明舵，邓丽，彭倩，姚春莉（400038重庆，第三军医大学西南医院妇产科） [摘要] 目的 了解短时受精对体外受精-胚胎移植（in- vitro fertilization and embryo transfer ,IVF-ET）结局的影响。方法 选择在本生殖医学中心接受常规IVF治疗的268个周期，通过一定条件限制将其分为短时受精（71个周期）和过夜受精（197个周期）2组，比较2组的正常受精（2PN）率、多精受精率、卵裂率、优质胚胎率及临床妊娠率。结果 短时受精组的受精2PN）率、多精受精率、卵裂率、优质胚胎率、临床妊娠率等与过夜受精组比较均无统计学差异P＞0.05±36.69）%明显高于过夜受精组（64.04±41.85）%（P＜Effects of short imsemination on IVF outcomes Qu Zongyin Wang Fengying Li Yuyan He Wei Yu Chiyang Li Mingduo Deng Li Peng Qian Yao Chunli (Department of Obstetric and Gynecology ,SouthWest Hospital ,Third Military Medical University, ChongQing,400038) [Abstract] Objective：To investigategamete (sperm-oocyte) co-incubation on outcomes in in- vitro fertilization and embryo transfer (IVF-ET). Methods:　A total of 268 IVF –ET cycles were included. They were divided with some condition into shortened insemination group(with 71 cycles) and overnight insemination group(with 197 cycles). The IVF outcomes were analyzed . Results : There was no significantly difference in the number of retrieved oocytes ,percentage of oocytes at MⅡ stage, Diploid fertilization rate( two pronuclei,2PN),polyspermy (2PN) rate, cleavage rates, high quality embryo rates and the pregnancy rates between shortened insemination group and overnight fertilization group. Conclusion: Short gamete co-incubation is reliable and efficacious . However, it could not improve embryo quality. [Key words] embryo quality； shortened insemination；overnight insemination in vitro fertilizati-embryo transfer (IVF-ET) Corresponding author:Wang Fengying,Tel:86-23 E-mail：fyw018@163.com近几年，国内外一些生殖中心通过短时受精方式进行体外受精（in-vitro fertilization，IVF），获得了较好的IVF结果[1]。但也有研究者发现短时受精不但不能改变IVF结局，还可明显增加多精受精率[2]。本中心也对部分IVF周期进行了短时受精，现将结果总结如下，以进一步探讨短时受精在IVF中的作用。 1 方法 1.1临床资料即受精方式的选择 选择2008年10月至2009年9月在本中心进行IVF治疗的268个周期。短时受精选择主要依据以下几个条件：卵子整体成熟度较好，授精前不需要延时培养者；精子参数较差，常规IVF存在一定风险，需要提前了解受精情况者；对卵子数量在14个以上的部分周期，将卵子一分为二，一部分进行短时受精，一部分进行常规过夜受精（配对资料）。对于卵子整体成熟度明显不够需