Capsaicin induces the production of IL 6 in human upper respiratory epithelial cells英文电子书.pdf

Life Sciences 80 (2007) 1592 – 1597 /locate/lifescie Capsaicin induces the production of IL-6 in human upper respiratory epithelial cells Nobuhiko Seki ⁎, Hideaki Shirasaki, Megumi Kikuchi, Tetsuo Himi Department of Otolaryngology, Sapporo Medical University School of Medicine, S1W16, Chuo-ku, Sapporo 060-8543, Japan Received 25 September 2006; accepted 18 January 2007 Abstract Capsaicin, a type of alkaloid and the pungent component of chili peppers, is used as a therapeutic drug against allergic rhinitis and also as an index of bronchial hypersensitivity. Capsaicin receptor (TRPV1) expression has been identified in non-neuronal cells as well as neuronal cells. In our previous study, both TRPV1 protein and its gene expression on nasal epithelial cells were confirmed by immunohistochemistry and RT- PCR, respectively. In order to clarify whether or not TRPV1 acts as a functional receptor, we examined the effects of capsaicin on the production of IL-6 from primary cultured human airway epithelial cells at both protein and mRNA levels. Human nasal epithelial cells (HNECs) and normal human bronchial/tracheal epithelial cells (NHBE cells) were stimulated with increasing concentrations of capsaicin and/or pretreatment with capsazepine (TRPV1 antagonist) at 37 °C. The supernatant and total RNA were collected at 0, 4, 12, 24 and 48 h after treatment. IL-6 concentration and the IL-6 mRNA level were evaluated by ELISA and real-time PCR, respectively. Capsaicin (10 nM– 10 μM) induced production of IL-6 from HNECs and NHBE cells and this effec