Contributions of citrate in redox potential maintenance and ATP production metabolic pathways and their regulation in Lactobacillus panis PM1英文电子书.pdf

Appl Microbiol Biotechnol (2013) 97:8693–8703 DOI 10.1007/s00253-013-5108-2 APPLIED MICROBIAL AND CELL PHYSIOLOGY Contributions of citrate in redox potential maintenance and ATP production: metabolic pathways and their regulation in Lactobacillus panis PM1 Tae Sun Kang Darren R. Korber Takuji Tanaka Received: 23 May 2013 /Revised: 7 July 2013 /Accepted: 8 July 2013 /Published online: 4 August 2013 # Springer-Verlag Berlin Heidelberg 2013 Abstract Lactobacillus panis PM1 belongs to the group III Keywords NADH reoxidation . Oxaloacetate heterofermentative lactobacilli and can utilize various decarboxylase . PocR . qRT-PCR NADH-reoxidizing routes (e.g., citrate, glycerol, and oxy- gen) according to environmental conditions. In this study, we investigated the ability of L. panis PM1 to produce succi- Introduction nate, acetate, and lactate via citrate utilization. Possible path- ways, as well as regulation, for citrate metabolism were Citrate is present in many natural products such as fruit, examined on the basis of the genome sequence data and vegetables, and milk and is commonly found as a natural metabolic profiles of L. panis PM1. The presence of citrate metabolite in living cells. Under aerobic conditions, citrate is led to the up-regulation, at the transcriptional level, of the always dissimilated via the tricarboxylic acid cycle (TCA genes encoding for citrate lyase, malate dehydrogenase, and cycle). Various bacterial fermentation pathways are also in- malic enzyme of the citrate pathways by 10- to 120-fold. The volved in citrate metabolism under anaerobic condition (Bott transcriptional regulator of the dha operon coding for glyc- 1997). Citrate metabolism has been extensively studied in erol dehydratase of L. panis PM1 repressed the expression of