Motif CXCC in nitrile hydratase activator is critical for NHase biogenesis in vivo英文电子书.pdf

FEBS 27705 FEBS Letters 553 (2003) 391^396 Motif CXCC in nitrile hydratase activator is critical for NHase biogenesis in vivo a;b ; b a;c a ; a Jun Lu , Yujuan Zheng , Hiromi Yamagishi , Masafumi Odaka , Masanari Tsujimura , Mizuo Maedaa , Isao Endoc aBioengineering Laboratory, The Institute of Physical and Chemical Research (RIKEN), Wako Shi, Saitama 351-0198, Japan b Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China c Department of Bioproduction Science, Faculty of Agriculture, Utsunomiya University, Minecho 350, Utsunomiya, Tochigi 321-8505, Japan Received 11 June 2003; revised 14 September 2003; accepted 16 September 2003 First published online 30 September 2003 Edited by Hans Eklund NHase operon consists of nhr2, nhr1, ami, nha1, nha2, and Abstract Nitrile hydratase (NHase) activator from Rhodococ- cus sp. N-771 is required for NHase functional expression. The nha3, six genes encoding NHase regulator 2, NHase regulator motif 73CXCC76 in the NHase activator sequence was here 1, a