Excitation Spectroscopy In Multispectral Optical Fluorescence Tomography Methodology Feasibility And Computer Simulation Studies英文电子书.pdf

IOP PUBLISHING PHYSICS IN MEDICINE AND BIOLOGY Phys. Med. Biol. 54 (2009) 4687–4704 doi:10.1088/0031-9155/54/15/004 Excitation spectroscopy in multispectral optical fluorescence tomography: methodology, feasibility and computer simulation studies 1,2 3 4 Abhijit J Chaudhari , Sangtae Ahn , Richard Levenson , Ramsey D Badawi2, Simon R Cherry1 and Richard M Leahy3 1 Department of Biomedical Engineering, University of California-Davis, Davis, CA 95616, USA 2 Department of Radiology, UC Davis Medical Center, Sacramento, CA 95817, USA 3 Signal and Image Processing Institute, University of Southern California, Los Angeles, CA 90089, USA 4 Cambridge Research Instruments, Inc., Woburn, MA 01801, USA E-mail: ajchaudhari@ and leahy@ Received 12 February 2009, in final form 11 June 2009 Published 10 July 2009 Online at /PMB/54/4687 Abstract Molecular probes used for in vivo optical fluorescence tomography (OFT) studies in small animals are typically chosen such that their emission spectra lie in the 680–850 nm wavelength range. This is because tissue attenuation in this spectral band is relatively low, allowing optical photons even from deep sites in tissue to reach the animal surface and consequently be detected by a CCD camera. The wavelength dependence of tissue optical properties within the 680–850 nm band can be exploited for emitted light by measuring fluorescent data via multispectral approache