Detection of Sarcocystis spp. in cattle (Bos taurus) and water buffaloes (Bubalus bubalis) in Iran by PCR–RFLP英文电子书.pdf

J Parasit Dis DOI 10.1007/s12639-014-0426-6 ORIGINAL ARTICLE Detection of Sarcocystis spp. in cattle (Bos taurus) and water buffaloes (Bubalus bubalis) in Iran by PCR–RFLP Hossein Hamidinejat • Mohammad Hossein Razi Jalali • Darioush Gharibi • Pedram Haddad Molayan Received: 10 November 2013 / Accepted: 13 January 2014 Indian Society for Parasitology 2014 Abstract Sarcocystis species are cyst-forming intracel- Introduction lular protozoan parasites. Cattle are mainly infected with Sarcocystis cruzi, Sarcocystis hominis and Sarcocystis The cyst-forming coccidian parasites, Sarcocystis spp. hirsuta. Water buffaloes are intermediate hosts for Sarco- (Apicomplexa: Sporozoa), have obligatory two-host life cystis fusiformis, Sarcocystis levinei (S. cruzi-like species), cycle involving carnivorous as definitive hosts and her- Sarcocystis dubeyi, Sarcocystis sinensis (S. hominis-like bivorous or omnivorous as intermediate hosts. These species) and Sarcocystis buffalonis (S. hirsuta- like spe- organisms cause sarcocystosis in domestic animals and cies). The aim of this study was Identification of Sarco- sometimes human and each intermediate and definitive cystis spp. in slaughtered cattle and water buffaloes in host may be infected with more than one Sarcocystis spe- Ahvaz, Khuzestan province by PCR-restriction fragment cies (Dubey et al. 1989). Cattle and water buffaloes are the length polymorphism. Meat inspection was done on 124 intermediate hosts of some important species of Sarco- cattle and 147 water buffaloes. From each animal tissue cystis, that is, they may harbor macro or micro sarcocystis samples (each 50 g) from heart, esophagus, diaphragm and on their muscl